Zinc transporter 8 autoantibodies in fulminant, acute-onset, and slow-onset patients with type 1 diabetes
| Autor: | Hironori Yamasaki, Nobuo Matsuura, Tsuyoshi Satoh, Genpei Kuriya, Hironaga Kuwahara, Kan Nakamura, Norio Abiru, Katsumi Eguchi, Masakazu Kobayashi, Yasuko Uchigata, Junnosuke Miura, Eiji Kawasaki |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Endocrinology Diabetes and Metabolism Fulminant Radioimmunoassay Zinc Transporter 8 Epitope Epitopes Endocrinology Acute onset Asian People Internal medicine Diabetes mellitus Internal Medicine medicine Humans Age of Onset Child Cation Transport Proteins Autoantibodies Type 1 diabetes business.industry Autoantibody Middle Aged medicine.disease Diabetes Mellitus Type 1 Amino Acid Substitution Female business Biomarkers |
| Zdroj: | Diabetes/Metabolism Research and Reviews. 27:895-898 |
| ISSN: | 1520-7552 |
| DOI: | 10.1002/dmrr.1269 |
| Popis: | The aim of this study was to determine the prevalence and role of autoantibodies to zinc transporter 8 (ZnT8A) in three forms (fulminant, acute-onset, and slow-onset) of Japanese patients with type 1 diabetes.One-hundred and ninety-six new-onset patients with type 1 diabetes were studied: 85 were fulminant, 81 acute-onset, and 30 slow-onset type 1 diabetes. ZnT8A were determined by radioimmunoassay using a hybrid ZnT8 carboxy-terminal construct (aa268-369) carrying 325Trp and 325Arg. Furthermore, ZnT8A epitopes were analysed using ZnT8 constructs incorporating the known aa325 variants (Trp, Arg, and Gln).ZnT8A were detected in 58% patients with acute-onset and 20% with slow-onset type 1 diabetes (p0.0005). In contrast, none of sera from fulminant type 1 diabetes were reactive to ZnT8 construct. Conversion of Arg or Trp to Gln at aa325 abolished reactivity in 59% of patients with an age of onset10 years, which was significantly higher than that in patients≤10 years of age (33%, p0.05).These results suggest that ZnT8A are an additional useful marker for acute-onset type 1 diabetes, but not a diagnostic marker for fulminant type 1 diabetes, and ZnT8A epitope recognition is different according to the onset age. |
| Databáze: | OpenAIRE |
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