Dissecting the stem cell niche with organoid models: an engineering-based approach
| Autor: | Zev J. Gartner, Robert J. Weber, Lyndsay M. Murrow |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Molecular composition Microfluidics Niche Review 02 engineering and technology Biology Models Biological 03 medical and health sciences Organoid Animals Humans Stem Cell Niche Molecular Biology Ecological niche Tissue Engineering 021001 nanoscience & nanotechnology Stem cell niche Cell biology Organoids 030104 developmental biology Cell culture Stem cell 0210 nano-technology Signal Transduction Developmental Biology Adult stem cell |
| Zdroj: | Development. 144:998-1007 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.140905 |
| Popis: | For many tissues, single resident stem cells grown in vitro under appropriate three-dimensional conditions can produce outgrowths known as organoids. These tissues recapitulate much of the cell composition and architecture of the in vivo organ from which they derive, including the formation of a stem cell niche. This has facilitated the systematic experimental manipulation and single-cell, high-throughput imaging of stem cells within their respective niches. Furthermore, emerging technologies now make it possible to engineer organoids from purified cellular and extracellular components to directly model and test stem cell-niche interactions. In this Review, we discuss how organoids have been used to identify and characterize stem cell-niche interactions and uncover new niche components, focusing on three adult-derived organoid systems. We also describe new approaches to reconstitute organoids from purified cellular components, and discuss how this technology can help to address fundamental questions about the adult stem cell niche. |
| Databáze: | OpenAIRE |
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