Prostaglandin D2 acts through the Dp2 receptor to influence male germ cell differentiation in the foetal mouse testis
| Autor: | Francis Poulat, Brigitte Boizet-Bonhoure, Kinya Nagata, Sabine Nidelet, Hiroyuki Hirai, Safdar Ujjan, Emeric Dubois, Kosuke Aritake, Yoshihiro Urade, Masataka Nakamura, Brigitte Moniot, Julien Champagne |
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| Přispěvatelé: | Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Department of Advanced Technology and Development, BML, Matoba, Osaka Bioscience Institute, Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Human Gene Sciences Center, Tokyo Medical and Dental University, Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Larose, Catherine |
| Rok vydání: | 2014 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Male Mouse Somatic cell Blotting Western Retinoic acid Fluorescent Antibody Technique [SDV.GEN] Life Sciences [q-bio]/Genetics Biology Real-Time Polymerase Chain Reaction Fibroblast growth factor Mice chemistry.chemical_compound Paracrine signalling Fetus Testis Germ cells medicine Animals Autocrine signalling Molecular Biology Cell Nucleus Mice Knockout Analysis of Variance [SDV.GEN]Life Sciences [q-bio]/Genetics Prostaglandin D2 Gene Expression Regulation Developmental RNA-Binding Proteins Cell Differentiation Embryonic testis 3. Good health Cell biology DNA-Binding Proteins medicine.anatomical_structure Mitotic arrest chemistry Differentiation Immunology Germ line development Germ cell Transcription Factors Developmental Biology |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2014, 141 (18), pp.3561-3571. ⟨10.1242/dev.103408⟩ Development (Cambridge, England), 2014, 141 (18), pp.3561-3571. ⟨10.1242/dev.103408⟩ |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.103408 |
| Popis: | Through intercellular signalling, the somatic compartment of the foetal testis is able to program primordial germ cells to undergo spermatogenesis. Fibroblast growth factor 9 and several members of the transforming growth factor β superfamily are involved in this process in the foetal testis, counteracting the induction of meiosis by retinoic acid and activating germinal mitotic arrest. Here, using in vitro and in vivo approaches, we show that prostaglandin D 2 (PGD 2 ), which is produced through both L-Pgds and H-Pgds enzymatic activities in the somatic and germ cell compartments of the foetal testis, plays a role in mitotic arrest in male germ cells by activating the expression and nuclear localization of the CDK inhibitor p21 Cip1 and by repressing pluripotency markers. We show that PGD 2 acts through its Dp2 receptor, at least in part through direct effects in germ cells, and contributes to the proper differentiation of male germ cells through the upregulation of the master gene Nanos2 . Our data identify PGD 2 signalling as an early pathway that acts in both paracrine and autocrine manners, and contributes to the differentiation of germ cells in the foetal testis. |
| Databáze: | OpenAIRE |
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