The effect of ouabain, meralluride and ethacrynic acid on respiration and glycolysis in kidney slices

Autor: Vernon D. Jones, Erwin J. Landon
Rok vydání: 1967
Předmět:
Zdroj: Biochemical Pharmacology. 16:2163-2169
ISSN: 0006-2952
DOI: 10.1016/0006-2952(67)90015-9
Popis: The effects of ouabain, meralluride, and ethacrynic acid on glycolysis and respiration of rat and rabbit kidney slices have been investigated. When slices were incubated under conditions favorable to glycolysis, ouabain and meralluride inhibited lactate formation with glucose or fructose as substrate. The inhibition of glycolysis was interpreted to be the result of a direct effect on the membrane ATPase system reducing the turnover rate of the cytoplasmic phosphoglycerate kinase reaction. Ouabain, meralluride, and ethacrynic acid inhibited O 2 uptake by the kidney slices. The inhibition of respiration by ouabain and meralluride could have been mediated through a decrease in ADP formation or by a reduced turnover of glycolytic intermediates. Ethacrynic acid inhibited respiration at lower levels than that required to inhibit glycolysis. Therefore, mitochondrial respiration could be the primary site of action of ethacrynic acid. These diuretic agents had no effect on glycolysis or respiration in slices that had been depleted of sodium with choline or lithium. Therefore the effects on energy metabolism in the presence of sodium would appear to be related to an effect on the sodium transport process.
Databáze: OpenAIRE