Crawling-induced floor dust resuspension affects the microbiota of the infant breathing zone
| Autor: | Pirkka V. Kirjavainen, Martin Täubel, Balamuralikrishna Jayaprakash, Sampo Saari, Anne Hyvärinen, Rauno Holopainen, Brandon E. Boor, Heidi Hyytiäinen, Kaarle Hämeri, Jorma Keskinen |
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| Přispěvatelé: | Tampere University, Physics, Department of Physics |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Air Microbiology Bacterial/genetics 010501 environmental sciences Crawling 01 natural sciences DNA Fungal/genetics PARTICULATE MATTER Fungi/classification RNA Ribosomal 16S Floors and Floorcoverings Ribosomal/genetics DNA Fungal Pcr analysis 16S rRNA gene sequencing 2. Zero hunger Inhalation exposure RNA Ribosomal 16S/genetics Fungal/genetics Microbiota Infant exposure Dust ASSOCIATION 3142 Public health care science environmental and occupational health Indoor/analysis Indoor microbial exposure CHILDRENS EXPOSURE QPCR DNA/methods DNA Bacterial/genetics qPCR Sequence Analysis DNA/methods Air Pollution Indoor Bacterial 16S rRNA Breathing lcsh:QR100-130 Sequence Analysis Bacteria/classification 16S/genetics Environmental Monitoring DNA Bacterial Microbiology (medical) Breathing zone animal structures DNA Ribosomal/genetics Biology DNA Ribosomal Microbiology 114 Physical sciences lcsh:Microbial ecology Dust/analysis EARLY-LIFE SAMPLING METHODS 03 medical and health sciences Animal science Microbial ecology Air Pollution Humans Air Pollution Indoor/analysis 0105 earth and related environmental sciences Ribosomal SEQUENCES Bacteria Research Fungi Infant Sequence Analysis DNA DNA 030104 developmental biology ASTHMA RNA ENDOTOXIN COMMUNITIES PCR ANALYSIS Particle resuspension |
| Zdroj: | Microbiome, Vol 6, Iss 1, Pp 1-12 (2018) Microbiome Hyytiäinen, H K, Jayaprakash, B, Kirjavainen, P V, Saari, S E, Holopainen, R, Keskinen, J, Hämeri, K, Hyvärinen, A, Boor, B E & Täubel, M 2018, ' Crawling-induced floor dust resuspension affects the microbiota of the infant breathing zone ', Microbiome, vol. 6, no. 1, 25 . https://doi.org/10.1186/s40168-018-0405-8 |
| Popis: | Background Floor dust is commonly used for microbial determinations in epidemiological studies to estimate early-life indoor microbial exposures. Resuspension of floor dust and its impact on infant microbial exposure is, however, little explored. The aim of our study was to investigate how floor dust resuspension induced by an infant’s crawling motion and an adult walking affects infant inhalation exposure to microbes. Results We conducted controlled chamber experiments with a simplified mechanical crawling infant robot and an adult volunteer walking over carpeted flooring. We applied bacterial 16S rRNA gene sequencing and quantitative PCR to monitor the infant breathing zone microbial content and compared that to the adult breathing zone and the carpet dust as the source. During crawling, fungal and bacterial levels were, on average, 8- to 21-fold higher in the infant breathing zone compared to measurements from the adult breathing zone. During walking experiments, the increase in microbial levels in the infant breathing zone was far less pronounced. The correlation in rank orders of microbial levels in the carpet dust and the corresponding infant breathing zone sample varied between different microbial groups but was mostly moderate. The relative abundance of bacterial taxa was characteristically distinct in carpet dust and infant and adult breathing zones during the infant crawling experiments. Bacterial diversity in carpet dust and the infant breathing zone did not correlate significantly. Conclusions The microbiota in the infant breathing zone differ in absolute quantitative and compositional terms from that of the adult breathing zone and of floor dust. Crawling induces resuspension of floor dust from carpeted flooring, creating a concentrated and localized cloud of microbial content around the infant. Thus, the microbial exposure of infants following dust resuspension is difficult to predict based on common house dust or bulk air measurements. Improved approaches for the assessment of infant microbial exposure, such as sampling at the infant breathing zone level, are needed. Electronic supplementary material The online version of this article (10.1186/s40168-018-0405-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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