Placental endothelin-converting enzyme-1 is decreased in preeclampsia
| Autor: | Judith U. Hibbard, Meredith Cruz, Jennifer McIntosh, Anna McCormick, Robert D. Blank, Xiaohu Wang, Jasmin Kristianto |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Endothelin converting enzyme 1 Placenta Down-Regulation 030204 cardiovascular system & hematology Endothelin-Converting Enzymes Immunofluorescence Article Preeclampsia Andrology 03 medical and health sciences 0302 clinical medicine Western blot Pre-Eclampsia Pregnancy Internal Medicine medicine Humans education Cellular localization education.field_of_study 030219 obstetrics & reproductive medicine medicine.diagnostic_test Chemistry Obstetrics and Gynecology Trophoblast Endothelial Cells medicine.disease medicine.anatomical_structure Case-Control Studies embryonic structures Female Chorionic Villi Stromal Cells Endothelin receptor Biomarkers |
| Zdroj: | Pregnancy Hypertens |
| Popis: | Endothelin-converting enzyme-1(ECE-1) is a key regulatory enzyme in the processing of endothelin-1 (ET-1). We quantified and localized ECE-1 in normal and preeclamptic placentas. Normal (n=6) and preeclamptic (n=6) placentas were serially sectioned for immunofluorescence (IF). Cell type specific markers identified endothelial, trophoblast, macrophage, smooth muscle, and fibroblast cells. Quantitative analyses were performed by western blot and ELISA. IF identified ECE-1 expression within the stroma and villous space. Cellular localization of ECE-1 was limited to endothelial membranes. There was significantly less ECE-1 in preeclamptic placentas, suggesting ECE-1 is important for proper regulation of ET-1 within the placenta. |
| Databáze: | OpenAIRE |
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