Isolation and characterization of novel bacteriophages as a potential therapeutic option for Escherichia coli urinary tract infections

Autor:	Ulises Hernández-Chiñas, Laura Belmont-Monroy, Gerardo Aparicio-Ozores, Carlos Alberto Eslava-Campos, Yerisaidy Ortega-García, Gerardo Erbey Rodea Montealegre, José Molina-López, Edgar González-Villalobos, Rosa María Ribas-Aparicio, José Luis Balcázar
Rok vydání:	2021
Předmět:	Phage therapy medicine.medical_treatment Myoviridae Multidrug resistance urologic and male genital diseases medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Siphoviridae 03 medical and health sciences medicine Humans Uropathogenic Escherichia coli Bacteriophages Escherichia coli Escherichia coli Infections 030304 developmental biology 0303 health sciences biology 030306 microbiology Biofilm General Medicine biology.organism_classification Multiple drug resistance Applied Microbial and Cell Physiology Lytic cycle Adherence Urinary Tract Infections Bacteria Biotechnology
Zdroj:	Applied Microbiology and Biotechnology
ISSN:	1432-0614 0175-7598
Popis:	Abstract Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC), whose impact can be exacerbated by multidrug-resistant (MDR) strains. Effective control strategies are, therefore, urgently needed. Among them, phage therapy represents a suitable alternative. Here, we describe the isolation and characterization of novel phages from wastewater samples, as well as their lytic activity against biofilm and adherence of UPEC to HEp-2 cells. The results demonstrated that phage vB_EcoM-phiEc1 (ϕEc1) belongs to Myoviridae family, whereas vB_EcoS-phiEc3 (ϕEc3) and vB_EcoS-phiEc4 (ϕEc4) belong to Siphoviridae family. Phages showed lytic activity against UPEC and gut commensal strains. Phage ϕEc1 lysed UPEC serogroups, whereas phages ϕEc3 and ϕEc4 lysed only UTI strains with higher prevalence toward the O25 serogroup. Moreover, phages ϕEc1 and ϕEc3 decreased both biofilm formation and adherence, whereas ϕEc4 was able to decrease adherence but not biofilm formation. In conclusion, these novel phages showed the ability to decrease biofilm and bacterial adherence, making them promising candidates for effective adjuvant treatment against UTIs caused by MDR UPEC strains. Key points Phage with lytic activity against MDR UPEC strains were isolated and characterized under in vitro conditions. A novel method was proposed to evaluate phage activity against bacterial adherence in HEp-2 cell.. Phages represent a suitable strategy to control infections caused by MDR bacteria.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65d202e8fa69b298c418453e02171cf5 https://doi.org/10.1007/s00253-021-11432-6 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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