Programmable CRISPR-responsive smart materials
| Autor: | Max A English, Helena de Puig, Angelo S. Mao, Luis R. Soenksen, Nicolaas M. Angenent-Mari, James J. Collins, Raphael V. Gayet, Peter Q. Nguyen |
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| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Institute for Medical Engineering & Science, Massachusetts Institute of Technology. Department of Mechanical Engineering, Massachusetts Institute of Technology. Synthetic Biology Center |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Cells CRISPR-Associated Proteins Acrylic Resins DNA Single-Stranded Nanoparticle Biocompatible Materials Nanotechnology Biosensing Techniques Smart material Permeability Polyethylene Glycols chemistry.chemical_compound Bacterial Proteins Tissue engineering Lab-On-A-Chip Devices CRISPR Fluidics DNA Cleavage Pathology Molecular Bioelectronics Endodeoxyribonucleases Multidisciplinary Tissue Engineering technology industry and agriculture Hydrogels DNA Cross-Linking Reagents chemistry Self-healing hydrogels Nanoparticles Ethylene glycol |
| Zdroj: | Prof. Collins via Howard Silver |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | Stimuli-responsive materials activated by biological signals play an increasingly important role in biotechnology applications. We exploit the programmability of CRISPR-associated nucleases to actuate hydrogels containing DNA as a structural element or as an anchor for pendant groups. After activation by guide RNA–defined inputs, Cas12a cleaves DNA in the gels, thereby converting biological information into changes in material properties. We report four applications: (i) branched poly(ethylene glycol) hydrogels releasing DNA-anchored compounds, (ii) degradable polyacrylamide-DNA hydrogels encapsulating nanoparticles and live cells, (iii) conductive carbon-black–DNA hydrogels acting as degradable electrical fuses, and (iv) a polyacrylamide-DNA hydrogel operating as a fluidic valve with an electrical readout for remote signaling. These materials allow for a range of in vitro applications in tissue engineering, bioelectronics, and diagnostics. Defense Threat Reduction Agency (Grant HDTRA1-14-1-0006) |
| Databáze: | OpenAIRE |
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