A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis
| Autor: | Fiamma Camargo, Naira C. Pesquero, Ricardo A. G. de Oliveira, Ronaldo C. Faria |
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| Přispěvatelé: | Universidade Federal de São Carlos (UFSCar), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2016 |
| Předmět: |
Paper
Analyte Microfluidics Low-cost diagnostics 02 engineering and technology Urine 01 natural sciences Biochemistry Analytical Chemistry Blood serum Lab-On-A-Chip Devices Environmental Chemistry Humans Paper-based analytical devices Spectroscopy Nitrites Detection limit Chromatography Filter paper Small volume Chemistry 010401 analytical chemistry Proteins Paper based Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Glucose Artificial urine 0210 nano-technology Point-of-care devices Hydrophobic and Hydrophilic Interactions Colorimetric detection |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1873-4324 |
| Popis: | Made available in DSpace on 2018-12-11T16:45:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-03-08 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This paper describes the fabrication of 2D and 3D microfluidic paper-based analytical devices (μPADs) for monitoring glucose, total protein, and nitrite in blood serum and artificial urine. A new method of cutting and sealing filter paper to construct μPADs was demonstrated. Using an inexpensive home cutter printer soft cellulose-based filter paper was easily and precisely cut to produce pattern hydrophilic microchannels. 2D and 3D μPADs were designed with three detection zones each for the colorimetric detection of the analytes. A small volume of samples was added to the μPADs, which was photographed after 15 min using a digital camera. Both μPADs presented an excellent analytical performance for all analytes. The 2D device was applied in artificial urine samples and reached limits of detection (LODs) of 0.54 mM, 5.19 μM, and 2.34 μM for glucose, protein, and nitrite, respectively. The corresponding LODs of the 3D device applied for detecting the same analytes in artificial blood serum were 0.44 mM, 1.26 μM, and 4.35 μM. Departamento de Química Universidade Federal de São Carlos Intituto de Química-UNESP, Av. Francisco Degni s/n Intituto de Química-UNESP, Av. Francisco Degni s/n CNPq: 140484/2013-2 FAPESP: 2015/19890-1 CNPq: 427727/2016-2 |
| Databáze: | OpenAIRE |
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