Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology
| Autor: | Santina Grazioli, Anna B. Ludi, Lynne Hendry, Donald P. King, Antonello Di Nardo, Anthony Relmy, Emiliana Brocchi, Tim Pollard, Clare Browning, P.L. Eblé, Aurore Romey, Lissie Henry |
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| Přispěvatelé: | Institute for Animal Health, the Pirbright Institute, Animal and Plant Health Agency [Addlestone, UK] (APHA), Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Wageningen BioVeterinary Research, Wageningen University and Research [Wageningen] (WUR), Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna 'Bruno Ubertini' (IZSLER), DEFRA (Department for Environment, Food Rural Affairs) SE1129SV3022UK Research & Innovation (UKRI)Biotechnology and Biological Sciences Research Council (BBSRC)BBS/E/I/00007035BBS/E/I/00007036 |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
Cattle Diseases serology Enzyme-Linked Immunosorbent Assay Viral Nonstructural Proteins Antibodies Viral Screen test Sensitivity and Specificity Virus Serology 0403 veterinary science 03 medical and health sciences inter-laboratory study Animals Medicine 030304 developmental biology 0303 health sciences Disease surveillance [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health General Veterinary biology business.industry foot-and-mouth disease virus Reproducibility of Results Outbreak 04 agricultural and veterinary sciences biology.organism_classification Virology 3. Good health Virology & Molecular Biology Virologie & Moleculaire Biologie Vaccination Foot-and-Mouth Disease biology.protein Cattle ELISA Antibody Foot-and-mouth disease virus Brief Communications business |
| Zdroj: | Journal of Veterinary Diagnostic Investigation, 32(6), 933-937 Journal of Veterinary Diagnostic Investigation 32 (2020) 6 Journal of Veterinary Diagnostic Investigation Journal of Veterinary Diagnostic Investigation, American Association of Veterinary Laboratory Diagnosticians, 2020, 32 (6), pp.933-937. ⟨10.1177/1040638720962070⟩ Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc |
| ISSN: | 1040-6387 |
| Popis: | International audience; Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance. |
| Databáze: | OpenAIRE |
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