Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes
| Autor: | Xiangjing Hu, Xue Feng, Yanmin Feng, Yujian Wu, Min Wang, Shangying Liao, Daoqin Zhang, Xiwen Lin, Longfei Ma, Xiuxia Wang, Zhen Li, Chunwei Zheng, Jian Chen, Si Wang, Chunsheng Han |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Invited Research Highlight Sertoli cells Cellular differentiation Retinoic acid Tretinoin Biology Biochemistry Article Mice 03 medical and health sciences chemistry.chemical_compound Meiosis spermatocytes retinoic acid Genetics medicine Animals Humans meiosis Spermatogenesis lcsh:QH301-705.5 Gametogenesis spermatogonial stem cells lcsh:R5-920 Stem Cells Cell Differentiation Cell Biology Sertoli cell Molecular biology Coculture Techniques Spermatogonia 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) chemistry Stem cell lcsh:Medicine (General) Developmental Biology medicine.drug |
| Zdroj: | Stem Cell Reports, Vol 7, Iss 1, Pp 80-94 (2016) Stem Cell Reports Asian Journal of Andrology |
| ISSN: | 2213-6711 |
| Popis: | Summary Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulated by RA were identified by RNA sequencing. RA in combination with pup Sertoli cell co-culture resulted in a higher induction efficiency of 28%. Comparisons in the transcriptomic profiles of the induced spermatogenic cells and the isolated ones revealed the progressive induction of the germ cells. Using this model, we showed that Stra8, Agpat3, Fam57a, Wdr91, and Sox30 contributed to the proliferation and meiosis initiation differentially. In conclusion, we have efficiently generated spermatocytes using an RA/pup Sertoli cell-based in vitro model and provided proof-of-concept evidence for its application in identifying genes involved in mammalian meiosis. Graphical Abstract Highlights • Retinoic acid (RA) is sufficient for the induction of spermatocytes • RA activates meiotic genes while repressing genes for SSC self-renewal • An in vitro model for meiosis based on RA and pup Sertoli cells is established • Genes for meiosis have been identified using the in vitro model In this article, Han and colleagues show that retinoic acid (RA), which regulates a large number of genes, is sufficient for inducing spermatocytes from cultured mouse spermatogonial stem cells. RA and Sertoli cell co-culture from pup mice constitute an in vitro meiosis model of higher efficiency. The authors further show that this model can be used to identify meiotic genes. |
| Databáze: | OpenAIRE |
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