Comparative Analysis of Listeria monocytogenes Plasmids and Expression Levels of Plasmid-Encoded Genes during Growth under Salt and Acid Stress Conditions

Autor:	Siyun Wang, Thomas Brenner, Patricia Hingston, Lisbeth Truelstrup Hansen
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	plasmid characterization Health Toxicology and Mutagenesis ATPase acid tolerance lcsh:Medicine Toxicology medicine.disease_cause Multicopper oxidase Microbiology 03 medical and health sciences Plasmid Listeria monocytogenes medicine NADH peroxidase Gene 030304 developmental biology 0303 health sciences salt tolerance biology 030306 microbiology Chemistry lcsh:R Wild type 3. Good health food safety Osmolyte plasmid gene expression biology.protein
Zdroj:	Toxins, Vol 11, Iss 7, p 426 (2019) Hingston, P, Brenner, T, Hansen, L T & Wang, S 2019, ' Comparative Analysis of Listeria monocytogenes Plasmids and Expression Levels of Plasmid-Encoded Genes during Growth under Salt and Acid Stress Condition ', Toxins, vol. 11, no. 7, 426 . https://doi.org/10.3390/toxins11070426 Toxins Volume 11 Issue 7
ISSN:	2072-6651
Popis:	Listeria monocytogenes strains are known to harbour plasmids that confer resistance to sanitizers, heavy metals, and antibiotics however, very little research has been conducted into how plasmids may influence L. monocytogenes&rsquo ability to tolerate food-related stresses. To investigate this, a library (n = 93) of L. monocytogenes plasmid sequences were compared. Plasmid sequences were divided into two groups (G1 and G2) based on a repA phylogeny. Twenty-six unique plasmid types were observed, with 13 belonging to each of the two repA-based groups. G1 plasmids were significantly (p < 0.05) smaller than G2 plasmids but contained a larger diversity of genes. The most prevalent G1 plasmid (57,083 bp) was observed in 26 strains from both Switzerland and Canada and a variety of serotypes. Quantitative PCR (qPCR) revealed a > 2-fold induction of plasmid-contained genes encoding an NADH peroxidase, cadmium ATPase, multicopper oxidase, and a ClpL chaperone protein during growth under salt (6% NaCl) and acid conditions (pH 5) and ProW, an osmolyte transporter, under salt stress conditions. No differences in salt and acid tolerance were observed between plasmid-cured and wildtype strains. This work highlights the abundance of specific plasmid types among food-related L. monocytogenes strains, the unique characteristics of G1 and G2 plasmids, and the possible contributions of plasmids to L. monocytogenes tolerance to food-related stresses.
Databáze:	OpenAIRE
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