lncRNA RP11-147L13.8 suppresses metastasis and chemo-resistance by modulating the phosphorylation of c-Jun protein in GBC
| Autor: | Houbao Liu, Zhihui Gao, Kun Fan, Han Liu, Bohao Zheng, Dexiang Zhang, Wentao Sun, Xiaoling Ni, Xiaojian Ni, Tao Suo, Jiwen Wang, Wenze Wan, Sheng Shen |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
Biology Metastasis gallbladder cancer chemo-resistance long non-coding RNAs medicine Pharmacology (medical) Gallbladder cancer RC254-282 Chemo resistance phosphorylation c-jun c-Jun Correction Cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease Gemcitabine eye diseases Oncology Tumor progression Cancer research Phosphorylation Molecular Medicine Original Article medicine.drug |
| Zdroj: | Molecular Therapy Oncolytics Molecular Therapy: Oncolytics, Vol 23, Iss, Pp 124-137 (2021) Molecular Therapy: Oncolytics, Vol 23, Iss, Pp 531-533 (2021) |
| ISSN: | 2372-7705 |
| Popis: | Long non-coding RNAs (lncRNAs) have been identified as critical contributors in tumor progression for many types of cancer. However, their functions in gallbladder cancer (GBC) have not been systematically clarified. In this study, the clinical significance, biological function, and underlying mechanism of lncRNA RP11-147L13.8 in GBC were investigated. The quantitative real-time PCR result indicated that lncRNA RP11-147L13.8 was found to be recurrently downregulated in GBC tumor samples. Kaplan-Meier analysis revealed that decreased lncRNA RP11-147L13.8 expression level was associated with poor survival of GBC patients (p = 0.025). Then, both in vitro and in vivo experiments elucidated that the overexpression of lncRNA RP11-147L13.8 suppressed the migration and invasion abilities of GBC cells and promoted the sensitivity to gemcitabine of GBC cells. Furthermore, we found that lncRNA RP11-147L13.8 physically interacted with c-Jun protein and decreased the phosphorylation on serine-73 (c-Jun-Ser73), which might cause the enhancement of the migration, invasion, and sensitivity to gemcitabine of GBC tumor cells. In conclusion, our study identified lncRNA RP11-147L13.8 as a promising prognostic indicator for patients with GBC, providing insights into the molecular pathogenesis of GBC. lncRNA RP11-147L13.8 is a potential therapeutic combination for gemcitabine in GBC treatment. Graphical abstract In this study, it was found that lncRNA RP11-147L13.8 could physically interact with c-Jun protein and decreased the phosphorylation on serine-73 (c-Jun-Ser73), which might cause the enhancement of the migration, invasion, and sensitivity to gemcitabine of GBC tumor cells. |
| Databáze: | OpenAIRE |
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