Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli
| Autor: | Daria Matyushkina, A. I. Zubov, V. G. Ladygina, Daria V. Evsyutina, Artem V. Eremeev, Daria V. Rakitina, Gleb Y. Fisunov, Peter L. Scherbakov, Olga Pobeguts |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Crohn’s disease propionic acid lcsh:QR1-502 Virulence medicine.disease_cause Microbiology lcsh:Microbiology Pathogenesis 03 medical and health sciences medicine Macrophage Escherichia coli Transcription factor 030304 developmental biology Original Research chemistry.chemical_classification 0303 health sciences Gastrointestinal tract 030306 microbiology Chemistry Crohn’s disease-associated adherent-invasive Escherichia coli Phenotype macrophages Propionate bacteria 2D electrophoresis |
| Zdroj: | Frontiers in Microbiology Frontiers in Microbiology, Vol 11 (2020) |
| ISSN: | 1664-302X |
| Popis: | Crohn's disease (CD) is a severe chronic immune-mediated granulomatous inflammatory disease of the gastrointestinal tract. The mechanisms of CD pathogenesis remain obscure. Metagenomic analysis of samples from CD patients revealed that several of them have the elevated level of Escherichia coli with adhesive-invasive phenotype (AIEC). Previously, we isolated an E. coli strain CD isolate ZvL2 from a patient with CD, which features AIEC phenotype. Here, we demonstrate that prolonged growth on propionate containing medium stimulates virulent properties of CD isolate ZvL2, while prolonged growth on glucose reduces these properties to levels indistinguishable from laboratory strain K-12 MG1655. Propionate presence also boosts the ability of CD isolate ZvL2 to penetrate and colonize macrophages. The effect of propionate is reversible, re-passaging of CD isolate on M9 medium supplemented with glucose leads to the loss of its virulent properties. Proteome analysis of CD isolate ZvL2 growth in medium supplemented with propionate or glucose revealed that propionate induces expression porins OmpA and OmpW, transcription factors PhoP and OmpR, and universal stress protein UspE, which were previously found to be important for macrophage colonization by enteropathogenic bacteria. |
| Databáze: | OpenAIRE |
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