Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells

Autor: Dmitry O. Traktuev, Jalees Rehman, Stephanie Merfeld-Clauss, Brian H. Johnstone, Carrie L. Pell, Constance J. Temm-Grove, Robert V. Considine, Jason E. Bovenkerk, Jingling Li, Keith L. March
Rok vydání: 2004
Předmět:
Pluripotent Stem Cells
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Stromal cell
Angiogenesis
Transplantation
Heterologous
Adipose tissue
Mice
Nude
Apoptosis
Mice
SCID
Biology
Transfection
chemistry.chemical_compound
Mice
Genes
Reporter
Ischemia
Mice
Inbred NOD
Transforming Growth Factor beta
Physiology (medical)
Internal medicine
medicine
Animals
Humans
Growth Substances
Cells
Cultured
Hepatocyte Growth Factor
Adipose-Derived Regenerative Cells
Endothelial Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Genetic Therapy
Stromal vascular fraction
Cell Hypoxia
Hindlimb
Vascular endothelial growth factor
Vascular endothelial growth factor A
Endocrinology
chemistry
Adipose Tissue
Culture Media
Conditioned
Cancer research
Hepatocyte growth factor
Fibroblast Growth Factor 2
Endothelium
Vascular
Stromal Cells
Cardiology and Cardiovascular Medicine
Cell Division
medicine.drug
Stem Cell Transplantation
Zdroj: Circulation. 109(10)
ISSN: 1524-4539
Popis: Background— The delivery of autologous cells to increase angiogenesis is emerging as a treatment option for patients with cardiovascular disease but may be limited by the accessibility of sufficient cell numbers. The beneficial effects of delivered cells appear to be related to their pluripotency and ability to secrete growth factors. We examined nonadipocyte stromal cells from human subcutaneous fat as a novel source of therapeutic cells. Methods and Results— Adipose stromal cells (ASCs) were isolated from human subcutaneous adipose tissue and characterized by flow cytometry. ASCs secreted 1203±254 pg of vascular endothelial growth factor (VEGF) per 10 6 cells, 12 280±2944 pg of hepatocyte growth factor per 10 6 cells, and 1247±346 pg of transforming growth factor-β per 10 6 cells. When ASCs were cultured in hypoxic conditions, VEGF secretion increased 5-fold to 5980±1066 pg/10 6 cells ( P =0.0016). The secretion of VEGF could also be augmented 200-fold by transfection of ASCs with a plasmid encoding VEGF ( P P P P Conclusions— Our experiments delineate the angiogenic and antiapoptotic potential of easily accessible subcutaneous adipose stromal cells by demonstrating the secretion of multiple potentially synergistic proangiogenic growth factors. These findings suggest that autologous delivery of either native or transduced subcutaneous ASCs, which are regulated by hypoxia, may be a novel therapeutic option to enhance angiogenesis or achieve cardiovascular protection.
Databáze: OpenAIRE
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