Chromatin-dependent repression of the Arabidopsis floral integrator genes involves plant specific PHD-containing proteins
| Autor: | José M. Martínez-Zapater, Laura Narro-Diego, Jose A. Jarillo, Manuel Piñeiro, Regla Bustos, Leticia López-González, Alfonso Mouriz |
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| Přispěvatelé: | Ministerio de Ciencia e Innovación (España) |
| Rok vydání: | 2014 |
| Předmět: |
Time Factors
Photoperiod Molecular Sequence Data Arabidopsis MADS Domain Proteins Flowers Plant Science Biology Methylation Histone Deacetylases Histones Histone H3 Gene Expression Regulation Plant Amino Acid Sequence Epigenetics Psychological repression Research Articles Regulator gene Homeodomain Proteins Genetics Sequence Homology Amino Acid Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Lysine fungi Gene Expression Regulation Developmental Nuclear Proteins food and beverages Acetylation Cell Biology biology.organism_classification Chromatin Histone Regulatory sequence Mutation biology.protein Protein Binding |
| Zdroj: | Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA Digital.CSIC. Repositorio Institucional del CSIC instname Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA RIUR. Repositorio Institucional de la Universidad de La Rioja RIUR: Repositorio Institucional de la Universidad de La Rioja Universidad de La Rioja (UR) |
| Popis: | © 2014 American Society of Plant Biologists. All rights reserved. The interplay among histone modifications modulates the expression of master regulatory genes in development. Chromatin effector proteins bind histone modifications and translate the epigenetic status into gene expression patterns that control development. Here, we show that two Arabidopsis thaliana paralogs encoding plant-specific proteins with a plant homeodomain (PHD) motif, SHORT LIFE (SHL) and EARLY BOLTING IN SHORT DAYS (EBS), function in the chromatin-mediated repression of floral initiation and play independent roles in the control of genes regulating flowering. Previous results showed that repression of the floral integrator FLOWERING LOCUS T (FT) requires EBS. We establish that SHL is necessary to negatively regulate the expression of SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1), another floral integrator. SHL and EBS recognize di- and trimethylated histone H3 at lysine 4 and bind regulatory regions of SOC1 and FT, respectively. These PHD proteins maintain an inactive chromatin conformation in SOC1 and FT by preventing high levels of H3 acetylation, bind HISTONE DEACETYLASE6, and play a central role in regulating flowering time. SHL and EBS are widely conserved in plants but are absent in other eukaryotes, suggesting that the regulatory module mediated by these proteins could represent a distinct mechanism for gene expression control in plants. This work was funded by projects of the Spanish Ministry of Science and Innovation (BIO2010-15589 and CSD2007-00057) to J.A.J. and M.P. |
| Databáze: | OpenAIRE |
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