Regional and molecular separation of the four bioactivities of 'tribulin'
| Autor: | Philip D. Evans, F.-Y. Pang, A. Doyle, Frank Hucklebridge, Angela Clow, Max W. Adlard |
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| Rok vydání: | 1998 |
| Předmět: |
Isatin
Male Monoamine Oxidase Inhibitors Amberlite chemistry.chemical_compound Adrenal Glands Animals Humans GABA-A Receptor Antagonists Intestine Large Rats Wistar Benzodiazepine receptor binding Aqueous solution biology Elution General Neuroscience Receptors GABA-A Rats Liver chemistry Biochemistry Organ Specificity biology.protein Female Monoamine oxidase B Methanol Monoamine oxidase A Spleen |
| Zdroj: | Neuroscience Letters. 240:29-32 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(97)00916-6 |
| Popis: | The endocoid tribulin has four known bioactivities: monoamine oxidase A and B inhibitory activities (MAO-AI and MAO-BI) and peripheral and central benzodiazepine receptor binding inhibitory activities (PBR-I and CBR-I). Analysis of the four bioactivities in rat tissue reveals regional dissociation. Most notably liver was particularly rich in PBR-I yet contained no detectable MAO-AI. In addition we have succeeded in separating the four activities from human urine. MAO-AI and MAO-BI have greater retention on charcoal than PBR-I and CBR-I when eluted step-wise with aqueous methanol. MAO-AI can be separated from MAO-BI and in addition PBR-I can be separated from CBR-I by step aqueous methanol elution from Amberlite XAD-4. Hence we present two lines of evidence that, contrary to the original view, tribulin is composed of a number of distinct molecular components. |
| Databáze: | OpenAIRE |
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