Cytotoxicity and Genotoxicity Induced by the Pesticide Profenofos on Cultured Human Peripheral Blood Lymphocytes

Autor:	G. Prabhavathy Das, Abjal Pasha Shaik, Kaiser Jamil
Rok vydání:	2006
Předmět:	Insecticides Cell Survival Health Toxicology and Mutagenesis Lymphocyte Biology Toxicology medicine.disease_cause chemistry.chemical_compound medicine Humans Lymphocytes Cytotoxicity Cells Cultured Chromosome Aberrations Pharmacology Chemical Health and Safety Dose-Response Relationship Drug Organothiophosphates Organophosphate Public Health Environmental and Occupational Health In vitro toxicology Trypan Blue General Medicine Molecular biology Solutions Comet assay medicine.anatomical_structure chemistry Toxicity Trypan blue Comet Assay Genotoxicity DNA Damage Mutagens
Zdroj:	Drug and Chemical Toxicology. 29:313-322
ISSN:	1525-6014 0148-0545
DOI:	10.1080/01480540600653093
Popis:	Profenofos is a broad-spectrum organophosphate insecticide and acaricide used widely for agricultural and household purposes. The aim of this investigation was to determine its toxicity profile in vitro, using lymphocytes from peripheral blood samples of healthy human donors. We found the IC(50) of profenofos to be 3.5 microM as measured by Trypan blue dye exclusion method. Chromosomal analyses of the metaphase plates of the samples treated with sublethal concentrations of profenofos revealed satellite associations and chromatid breaks and gaps, indicating its effect on chromosomes. The results were further supported by comet assay, where single-strand breaks in DNA were observed as comet tail lengths. The results were statistically significant (p0.01, ANOVA). Hence, it may be proposed that in vitro assays like the comet assay and chromosomal aberrations test, which indicate genetic damage, could be used to study the effect of organophosphorus pesticide poisoning in humans.
Databáze:	OpenAIRE
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