Clinical and immunological implications of increase in CD208+ dendritic cells in tonsils of patients with immunoglobulin A nephropathy
| Autor: | Osamu Hotta, Hiroo Kumagai, Kojiro Yamamoto, Akihiro Shiotani, Hideyuki Shimazaki, Hiroshi Nagura, Seiichi Tamai, Takeshi Matsunobu, Hanako Takechi, Yutaka Sakurai, Naoki Oshima, Takashi Oda |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male glomerular crescent Pathology medicine.medical_specialty Blotting Western Palatine Tonsil Kidney Real-Time Polymerase Chain Reaction urologic and male genital diseases Palatine tonsil Flow cytometry Immunoenzyme Techniques Basic Science stomatognathic system medicine Humans RNA Messenger tonsillectomy Transplantation integumentary system medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry Lysosome-Associated Membrane Glycoproteins Glomerulonephritis IGA Glomerulonephritis Dendritic Cells Original Articles IgA nephropathy Flow Cytometry medicine.disease CD208 Neoplasm Proteins medicine.anatomical_structure Real-time polymerase chain reaction Nephrology Case-Control Studies Tonsil Immunology Immunohistochemistry Female Renal biopsy business |
| Zdroj: | Nephrology Dialysis Transplantation |
| ISSN: | 1460-2385 0931-0509 |
| DOI: | 10.1093/ndt/gft399 |
| Popis: | Background The therapeutic effect of tonsillectomy for immunoglobulin A nephropathy (IgAN) has been widely recognized, but the mechanism by which tonsillar immunity leads to glomerulonephritis has been unclear. We investigated subtypes and localization of dendritic cells (DCs) in tonsils and looked for relationships between the tonsillar DCs and the clinical features and renal histological changes of patients with IgAN. Methods We examined tonsils from 33 IgAN patients, using as control tonsillar specimens from subjects without glomerulonephritis. Five distinct markers of DCs (CD303, CD1c, CD209, CD208 and CD1a) were analyzed by immunohistochemistry and flow cytometry. The mRNA levels of these DC markers were evaluated using real-time polymerase chain reaction. The clinical data and histological results obtained evaluating renal biopsy tissues were statistically compared with immunological data. Results Of the five subtypes of DCs, CD208+ DCs were significantly increased in the tonsils of IgAN patients compared with that of controls. Furthermore, the number of CD208+ DCs in the tonsils was positively and linearly correlated with the proportion of crescentic glomeruli in renal biopsy tissues and with the urinary protein level. Only few CD208+ cells, however, were found in the kidney biopsy specimens of IgAN patients. Conclusions These observations suggest that increased CD208+ DCs in tonsils may play a directive role in the pathogenesis of IgAN. The present results support the therapeutic significance of tonsillectomy for IgAN patients. |
| Databáze: | OpenAIRE |
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