Effect of a BIOcocktail on the Immune Response at the Early Postoperative Period in Mice

Autor:	Gennady Smirnov, Alexander Zeidel, Benzion Beilin, Hanna Bessler, Alexander Kalinkovich, Israel Z. Yardeni
Rok vydání:	2005
Předmět:	medicine.medical_specialty Lipopolysaccharide T-Lymphocytes medicine.medical_treatment Period (gene) Mice chemistry.chemical_compound Immune system Laparotomy Internal medicine medicine Splenocyte Animals Postoperative Period Cell Proliferation B-Lymphocytes biology business.industry Probiotics Immunity Interleukin-12 Cytokine Endocrinology chemistry Concanavalin A Surgical Procedures Operative biology.protein Surgery business Thymidine
Zdroj:	Journal of Investigative Surgery. 18:143-149
ISSN:	1521-0553 0894-1939
DOI:	10.1080/08941930590956200
Popis:	Since human subjects and laboratory animals may develop impaired immune response during surgery and the postoperative period, efforts have been made to preserve normal immune functions following surgery by the administration of nutritional supplements and probiotics. The present study was designed to examine the effect of a new nutritional supplement, BIOcocktail, on immune parameters in mice exposed to surgery. Forty mice were assigned to 4 groups containing 10 animals each. Two control groups (with and without subsequent sham laparotomy) were given tap water for 45 min every day for 2 weeks. The remaining 2 groups, with and without laparotomy, received BIOcocktail given orally for the same period of time. The proliferative response of splenic cells (splenocytes) stimulated with phytohemagglutinin (PHA), concanavalin A (Con A) and lipopolysaccharide (LPS) was determined by [3H]thymidine uptake. Cytokine levels were measured in splenocyte supernatants and sera using enzyme-linked immunosorbent assay (ELISA) kits. Natural killer cell activity of splenocytes was evaluated by 51Cr-release assay. Laparotomy, without BIOcocktail administration, was followed by a decreased proliferative response of splenocytes to PHA, Con A, and LPS and an increase in interleukin (IL)-6 serum level. In addition, a decreased secretion of IL-1beta, IL-12 and tumor necrosis factor (TNF)-alpha by the splenocytes was observed. Mice treated with BIOcocktail before laparotomy maintained a preoperative level of splenocyte proliferative response and serum concentrations of IL-12. It is concluded that BIOcocktail administered to mice for 2 weeks before operation resulted in the preservation of T- and B-cell proliferative response to mitogens and in the prevention of postoperative decrease in IL-12 serum level.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65baaa702068f13c25eca1cd6877abd5 https://doi.org/10.1080/08941930590956200 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.