Lipid-rich enteral nutrition regulates mucosal mast cell activation via the vagal anti-inflammatory reflex
| Autor: | M'hamed Hadfoune, Cathy Cailotto, Jacco J. de Haan, Tim Lubbers, Wouter J. de Jonge, Akihiko Ito, Jan Greve, Kaatje Lenaerts, Isabelle Verbaeys, Michael J. Skynner, Caroline M. Hodin, Jan van der Vliet, Wim A. Buurman |
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| Přispěvatelé: | Tytgat Institute for Liver and Intestinal Research, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Gastroenterology and Hepatology, RS: NUTRIM - R2 - Gut-liver homeostasis, Surgery |
| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Physiology Histamine Antagonists Inflammation Nicotinic Antagonists Cholinergic Agonists Receptors Nicotinic Biology Cell Degranulation Cell Line Mice Chymases Enteral Nutrition Physiology (medical) Internal medicine Reflex medicine Animals Mast Cells Intestinal Mucosa Receptor Immunity Mucosal Vagotomy Proximal Gastric Cholinergic anti-inflammatory pathway Mice Knockout Hepatology Vagovagal reflex Gastroenterology Cardiovascular diseases Tissue engineering and pathology [NCEBP 14] Vagus Nerve Dietary Fats beta-N-Acetylhexosaminidases Receptor Cholecystokinin A Vagus nerve Mice Inbred C57BL Interleukin 33 Disease Models Animal Nicotinic acetylcholine receptor Endocrinology medicine.symptom |
| Zdroj: | American Journal of Physiology : Gastrointestinal and Liver Physiology, 305, 5, pp. G383-91 American journal of physiology. Gastrointestinal and liver physiology, 305(5), G383-G391. American Physiological Society American Journal of Physiology : Gastrointestinal and Liver Physiology, 305, G383-91 American Journal of Physiology-Gastrointestinal and Liver Physiology, 305(5), G383-G391. American Physiological Society |
| ISSN: | 0193-1857 |
| Popis: | Nutritional stimulation of the cholecystokinin-1 receptor (CCK-1R) and nicotinic acetylcholine receptor (nAChR)-mediated vagal reflex was shown to reduce inflammation and preserve intestinal integrity. Mast cells are important early effectors of the innate immune response; therefore modulation of mucosal mast cells is a potential therapeutic target to control the acute inflammatory response in the intestine. The present study investigates intestinal mast cell responsiveness upon nutritional activation of the vagal anti-inflammatory reflex during acute inflammation. Mucosal mast cell degranulation was induced in C57/Bl6 mice by administration of Salmonella enterica LPS. Lipid-rich enteral feeding prior to LPS significantly decreased circulatory levels of mouse mast cell protease at 30 min post-LPS compared with isocaloric low-lipid nutrition or fasting. CCK-1R blockage reversed the inhibitory effects of lipid-rich feeding, whereas stimulation of the peripheral CCK-1R mimicked nutritional mast cell inhibition. The effects of lipid-rich nutrition were negated by nAChR blockers chlorisondamine and α-bungarotoxin and vagal intestinal denervation. Accordingly, release of β-hexosaminidase by MC/9 mast cells following LPS or IgE-ovalbumin complexes was dose dependently inhibited by acetylcholine and nicotine. Application of GSK1345038A, a specific agonist of the nAChR α7, in bone marrow-derived mast cells from nAChR β2−/− and wild types indicated that cholinergic inhibition of mast cells is mediated by the nAChR α7 and is independent of the nAChR β2. Together, the present study reveals mucosal mast cells as a previously unknown target of the nutritional anti-inflammatory vagal reflex. |
| Databáze: | OpenAIRE |
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