| Autor: |
Luís Fernando de Macedo Brígido, Mariza G. Morgado, José Carlos Couto-Fernandez, M. Ueda, Bernardo Galvão-Castro, Carlos Alberto Ramos, Cassimiro Oliveira, Vera Bongertz, M. Rossini, M. I. Linhares de Carvalho, M. Caseiro, D. C. Bou-Habib, D. Greco, P. J. N. Chequer, Monick Lindenmeyer Guimarães, Amilcar Tanuri, P. C. Ferreira, E Sabino, Saladin Osmanov |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
Scopus-Elsevier |
| ISSN: |
1525-4135 |
| DOI: |
10.1097/00126334-200002010-00011 |
| Popis: |
The Brazilian Network for HIV Isolation and Characterization was established for the surveillance of HIV variability in Brazil. Here the authors report characterization of HIV strains and virus-specific immune responses from 35 clinical samples collected from 3 potential HIV vaccine sites. 3 genetic subtypes of HIV-1 were identified by heteroduplex mobility assay (HMA): B (in 82.9% of the samples) F (in 14%.3) and C (in 2.9%). Phylogenetic analysis based on the C2V3/env DNA sequence from all 25 specimens examined was 100% concordant with HMA results. Four variants of subtype B with different tetrapeptides at the tip of the V3 loop were found: the GPGR motif (North American) the GWGR motif (Brazilian B”) and two minor variants GFGR and GPGS as previously detected. No significant association was found between HIV-1 subtypes and the mode of transmission or biologic properties of HIV-isolates (derived from 88.6% of the specimens). Only 5 of 16 isolates studied were neutralized by the autologous sera. Consistent with previous results no relation between viral subtype and peptide enzyme-linked immunosorbent assay (ELISA) seroreactivity or neutralization was evident. The study also demonstrated the effectiveness of the collaborative approach followed by Brazilian scientists when addressing a complex subject such as HIV variability. (author’s) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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