An investigation in the remineralization and acid resistant characteristics of nanohydroxyapatite produced from eggshell waste via mechanochemistry
| Autor: | Sandile Cromwell Mkhize, Ntokozo Lihlithemba Ndumiso Mabaso, Stanley Chibuzor Onwubu, Phumlane Selby Mdluli, Surendra Thakur, Deneshree Naidoo |
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| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
lcsh:Biotechnology Biomedical Engineering Biophysics Acid resistance Bioengineering Biocompatible Materials 02 engineering and technology Biomaterials 03 medical and health sciences Egg Shell Mice 0302 clinical medicine X-Ray Diffraction Mechanochemistry lcsh:TP248.13-248.65 Spectroscopy Fourier Transform Infrared Animals Eggshell Remineralisation Chemistry 030206 dentistry General Medicine Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Models Dental Nanostructures Durapatite Chemical engineering Tooth Remineralization NIH 3T3 Cells 0210 nano-technology |
| Zdroj: | Journal of Applied Biomaterials & Functional Materials, Vol 18 (2020) |
| ISSN: | 2280-8000 |
| Popis: | Objectives: This study focuses on the properties of nanohydroxyapatite (nHAp) in terms of remineralization and acid resistance. The nHAp were produced from waste eggshells via the mechanochemistry process. Materials and methods: The characterization was based on Fourier Transform Spectroscopy, X-ray diffraction, Field Scanning Electron Microscope (FESEM), and High-Resolution Electron Microscope to determine the surface morphology of the nHAp. The acid and remineralization properties were evaluated using bovine enamel and dentine models ( n = 5) while the buffering properties against acids were studied using a pH meter. The biocompatibility of the produce nHAp was assessed in vitro against NIH 3T3. Results: The XRD and FTIR results confirm that nHAp were successfully produced from eggshell waste after 5 h of milling. The HRTEM reveals a semi-sphere morphology with an average dimension of 9 to 20 nm. The buffering test suggests that nHAp were highly effective in neutralizing common dietary acids. Also, the nHAp exhibits outstanding remineralization and occluding properties. The cytotoxicity assay suggests that the nHAp had a low toxicity. Conclusion: The study concludes that using eggshell waste to produce nHAp will help in waste management and at the same time, provide valuable biomaterial for the treatment of tooth sensitivity. |
| Databáze: | OpenAIRE |
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