Strain-induced collagen organization at the micro-level in fibrin-based engineered tissue constructs
Autor: | Fpt Frank Baaijens, Cvc Carlijn Bouten, Frans Kanters, Nicky de Jonge |
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Přispěvatelé: | Soft Tissue Biomech. & Tissue Eng., Electrical Engineering |
Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
Micro level
Fibrin Microscopy Confocal Materials science Tissue Engineering Tissue Scaffolds biology Strain (chemistry) Models Cardiovascular Biomedical Engineering Biomechanics Matrix (biology) Time-Lapse Imaging Biomechanical Phenomena Tissue culture Tissue engineering biology.protein Humans Collagen Stress Mechanical Myofibroblasts Myofibroblast Biomedical engineering |
Zdroj: | Annals of Biomedical Engineering, 41(4), 763-774. Springer |
ISSN: | 0090-6964 |
Popis: | Full understanding of strain-induced collagen organization in complex tissue geometries to create tissues with predefined collagen architecture has not been achieved. This is mainly due to our limited knowledge of collagen remodeling in developing tissues. Here we investigate strain-induced collagen (re)organization in fibrin based engineered tissues using nondestructive time-lapse imaging. The tissues start from a biaxially constrained myofibroblast-populated fibrin gel and are used to study: (A) remodeling from a static equi-biaxial loading condition to static uniaxial loading; and (B) remodeling of a biaxially constrained tissue under uniaxial cyclic straining before and after a change in strain direction. Under static conditions, collagen oriented parallel to the direction of strain, whereas under cyclic conditions the orientation in the constrained tissue was perpendicular to the direction of strain. It is concluded that due to the biaxial constraints the uniaxially, cyclically strained cells can exert forces in two directions and strain shield themselves. A subsequent change in the direction of cyclic straining resulted in a rapid reorientation of collagen at the tissue surface. Reorientation was significantly slower in deeper tissue layers, where tissue remodeling was dominated by contact guidance provided by the endogenous matrix. These findings emphasize the relevance of achieving a functional collagen organization right from the start of tissue culture. |
Databáze: | OpenAIRE |
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