Distinct Viral Populations Differentiate and Evolve Independently in a Single Perennial Host Plant
| Autor: | Gérard Labonne, Chiraz Jridi, Jean-François Martin, Véronique Marie-Jeanne, Stéphane Blanc |
|---|---|
| Přispěvatelé: | UMR INRA / ENSAM / CIRAD : Biologie et Génétique des Interactions Plantes / Parasite pour la Protection Intégrée, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Montpellier (ENSA M), Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Rok vydání: | 2006 |
| Předmět: |
0106 biological sciences
viruses Immunology Population Metapopulation PLUM POX VIRUS Biology 01 natural sciences Microbiology Virus Evolution Molecular 03 medical and health sciences Virology Genetic variation education Clade Polymorphism Single-Stranded Conformational 030304 developmental biology Genetics 0303 health sciences education.field_of_study Genetic diversity BIOLOGIE DES POPULATIONS Reverse Transcriptase Polymerase Chain Reaction Host (biology) Genetic Variation Sequence Analysis DNA VIROLOGIE Haplotypes Genetic Diversity and Evolution Viral replication Insect Science [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology RNA Viral Prunus 010606 plant biology & botany |
| Zdroj: | Journal of Virology Journal of Virology, American Society for Microbiology, 2006, 80 (5), pp.2349-2357. ⟨10.1128/JVI.80.5.2349-2357.2006⟩ |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.80.5.2349-2357.2006 |
| Popis: | The complex structure of virus populations has been the object of intensive study in bacteria, animals, and plants for over a decade. While it is clear that tremendous genetic diversity is rapidly generated during viral replication, the distribution of this diversity within a single host remains an obscure area in this field of science. Among animal viruses, only Human immunodeficiency virus and Hepatitis C virus populations have recently been thoroughly investigated at an intrahost level, where they are structured as metapopulations, demonstrating that the host cannot be considered simply as a “bag” containing a homogeneous or unstructured swarm of mutant viral genomes. In plants, a few reports suggested a possible heterogeneous distribution of virus variants at different locations within the host but provided no clues as to how this heterogeneity is structured. Here, we report the most exhaustive study of the structure and evolution of a virus population ever reported at the intrahost level through the analysis of a Prunus tree infected by Plum pox virus for over 13 years following a single inoculation event and by using analysis of molecular variance at different hierarchical levels combined with nested clade analysis. We demonstrate that, following systemic invasion of the host, the virus population differentiates into several distinct populations that are isolated in different branches, where they evolve independently through contiguous range expansion while colonizing newly formed organs. Moreover, we present and discuss evidence that the tree harbors a huge “bank” of viral clones, each isolated in one of the myriad leaves. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|