Two Cases of Natural Infection of Dengue-2 Virus in Bats in the Colombian Caribbean
| Autor: | Alfonso Calderón, Camilo Guzmán, Víctor Castañeda, Teresa Oviedo-Socarras, Salim Mattar, Virginia Rodríguez, Luiz Tadeu Moraes Figueiredo |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Serotype 030231 tropical medicine lcsh:Medicine Spleen Dengue virus medicine.disease_cause Arbovirus Article Virus Dengue fever 03 medical and health sciences 0302 clinical medicine flavivirus MORCEGOS medicine antibodies General Immunology and Microbiology biology lcsh:R Public Health Environmental and Occupational Health biology.organism_classification medicine.disease Virology zoonoses Flavivirus arbovirus 030104 developmental biology Infectious Diseases medicine.anatomical_structure immunohistochemistry pathology biology.protein Antibody |
| Zdroj: | Tropical Medicine and Infectious Disease; Volume 6; Issue 1; Pages: 35 Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Tropical Medicine and Infectious Disease, Vol 6, Iss 35, p 35 (2021) Tropical Medicine and Infectious Disease |
| ISSN: | 2414-6366 |
| Popis: | Dengue, a mosquito-borne zoonotic disease, is the most common vector-borne disease in tropical and subtropical areas. In this study, we aim to demonstrate biological evidence of dengue virus infection in bats. A cross-sectional study was carried out in the departments of Cordoba and Sucre, Colombia. A total of 286 bats were captured following the ethical protocols of animal experimentation. The specimens were identified and euthanized using a pharmacological treatment with atropine, acepromazine and sodium pentobarbital. Duplicate samples of brain, heart, lung, spleen, liver, and kidney were collected with one set stored in Trizol and the other stored in 10% buffered formalin for histopathological and immunohistochemical analysis using polyclonal antibodies. Brain samples from lactating mice with an intracranial inoculation of DENV-2 were used as a positive control. As a negative control, lactating mouse brains without inoculation and bats brains negative for RT-PCR were included. Tissue sections from each specimen of bat without conjugate were used as staining control. In a specimen of Carollia perspicillata captured in Ayapel (Cordoba) and Phylostomus discolor captured in San Carlos (Cordoba), dengue virus was detected, and sequences were matched to DENV serotype 2. In bats RT-PCR positive for dengue, lesions compatible with viral infections, and the presence of antigens in tissues were observed. Molecular findings, pathological lesions, and detection of antigens in tissues could demonstrate viral DENV-2 replication and may correspond to natural infection in bats. Additional studies are needed to elucidate the exact role of these species in dengue epidemics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|