The demonstration of adenosinetriphosphatase activity with the electronmicroscope
| Autor: | A. E. F. H. Meijer, J. P. Persijn, J. C. H. de Man, W. Th. Daems |
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| Rok vydání: | 1961 |
| Předmět: |
Adenosine Triphosphatases
chemistry.chemical_classification Microscopy Ion Transport Histology Sucrose Chromatography Magnesium Hydrolysis chemistry.chemical_element Cell Biology Tartrate Adenosine Medical Laboratory Technology chemistry.chemical_compound Enzyme chemistry Biochemistry medicine Alkaline phosphatase Molecular Biology Magnesium ion Incubation medicine.drug |
| Zdroj: | Histochemie. 2:372-382 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/bf00848042 |
| Popis: | A new method for the demonstration of adenosine triphosphatase (ATP'ase) activity in tissue blocks with the electron-microscope has been developed. This method consists of short fixation (2–3 minutes,ph 7.3) in a OsO4-Tris-HCl mixture, incubation with a medium containing Tris-HCl buffer, tartrate, lead and magnesium ions, ATP and sucrose (ph 6.8) and routine postfixation. The method combines satisfactory histochemical specificity with good preservation of tissue fine structure. Estimations of enzymatic activity of ATP'ase and alkaline phosphatase, as observed with the electronmicroscope, were compared with activity determinations in tissue homogenates. The occurence of a positive histochemical reaction in swollen mitochondria is discussed. |
| Databáze: | OpenAIRE |
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