Vascular calcification: expression patterns of the osteoblast-specific gene core binding factor alpha-1 and the protective factor matrix Gla protein in human atherogenesis
| Autor: | J. M. Neele, A.L.J.J. Bronckers, C. J. M. De Vries, Hans Pannekoek, Marten A. Engelse |
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| Přispěvatelé: | Orale Celbiologie (OUD, ACTA), Other departments |
| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty Endothelium Arteriosclerosis Physiology Biology Core binding factor Muscle Smooth Vascular Physiology (medical) Matrix gla protein medicine Humans RNA Messenger Cells Cultured In Situ Hybridization Extracellular Matrix Proteins Reverse Transcriptase Polymerase Chain Reaction Macrophages Monocyte Calcium-Binding Proteins Core Binding Factors Calcinosis nutritional and metabolic diseases Osteoblast medicine.disease Immunohistochemistry Neoplasm Proteins Cell biology Endothelial stem cell RUNX2 medicine.anatomical_structure Case-Control Studies biology.protein Endothelium Vascular Cardiology and Cardiovascular Medicine Transcription Factors Calcification |
| Zdroj: | Engelse, M A, Neele, J M, Bronckers, A L J J, Pannekoek, H & de Vries, C J M 2001, ' Vascular calcification: expression patterns of the osteoblast-specific gene core binding factor alpha-1 and the protective factor matrix Gla protein in human atherogenesis ', Cardiovascular Research, vol. 52, pp. 281-289 . https://doi.org/10.1016/S0008-6363(01)00375-3 Cardiovascular Research, 52, 281-289. Oxford University Press Cardiovascular research, 52, 281-289. Oxford University Press Cardiovascular research, 52(2), 281-289. Oxford University Press |
| ISSN: | 0008-6363 |
| DOI: | 10.1016/S0008-6363(01)00375-3 |
| Popis: | Objective: Increasing evidence suggests that vascular calcification is a regulated process. We studied the vascular expression pattern of a key factor in mineralization and a counteracting, protective factor. Based on the phenotype of null mice, Core binding factor α-1 (Cbfa-1) plays a pivotal role in bone formation, whereas Matrix Gla Protein (MGP) is a potent inhibitor of vascular calcification. Methods: We investigated the expression of MGP and Cbfa-1 in cultured, human monocytic cells, endothelial cells and smooth muscle cells (SMC), as well as in normal and atherosclerotic vessel specimens. Results: In cultured cells MGP is expressed in endothelial cells and SMC, whereas Cbfa-1 mRNA is predominantly present in macrophages and to a lesser extent in SMC. In the normal vessel wall MGP expression is high at the luminal side and declines toward the center of the media, whereas Cbfa-1 is absent. Moderate, diffuse calcification of the aorta media was observed only in those regions where MGP is low or absent. In atherosclerotic lesions MGP is expressed in endothelial cells and SMC that form fibrous caps, but is never present in macrophages. Cbfa-1 is synthesized in regions without MGP, it is associated with calcified areas and Cbfa-1 may be considered a marker for osteoprogenitor-like cells in the vessel wall. Conclusions: Our observations on MGP expression confirm and extend published data and are consistent with a protective function of MGP. Cbfa-1 expression is absent in normal medial SMC and co-localizes with neointimal macrophages and focal calcifications. |
| Databáze: | OpenAIRE |
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