PRODUCTION OF MEDIUM CHAIN LENGTH POLYHYDROXYALKANOATES FROM Cupriavidus necator WITH BEESWAX HYDROLYZATES AS CARBON SOURCE
| Autor: | Jorge Noel Gracida-Rodríguez, Samuel Quintanar-Gómez, Evelyn Zamuido-Pérez, Genaro Vargas-Hernández, Arturo Abreu-Corona, Alejandro Téllez-Jurado |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
PHA Cupriavidus necator chemistry.chemical_element Biomass mcl Polyhydroxyalkanoates Beeswax DSC 03 medical and health sciences chemistry.chemical_compound Ciencias de la Tierra Ammonium Waste Management and Disposal biology Comonomer biology.organism_classification Pollution Nitrogen NMR 030104 developmental biology chemistry visual_art visual_art.visual_art_medium fed Carbon Nuclear chemistry |
| Zdroj: | Universidad Autónoma Metropolitana UAM Redalyc-UAM Revista Internacional de Contaminación Ambiental (México) Num.3 Vol.34 |
| Popis: | This study demonstrates that using beeswax hydrolyzates (Hw), short- or medium-chain-length polyhydroxyalkanoates (scl-mcl-PHA) can be obtained by a three stages fed-batch fermentation system. The fed batch fermentations were conducted with a strain of Cupriavidus necator . Glucose and ammonium sulphate as carbon and nitrogen sources, respectively, were used in a first and second culture stages. In the third stage (production of scl-mcl-PHA) a fresh medium was fed with Hw as a carbon source at a concentration of 5 g/L, obtaining 8.91 g/L of intracellular scl-mcl-PHA and 13.64 g/L of biomass, which resulted in 65.3 % w/w of scl-mcl-PHA. Using nuclear magnetic resonance, the presence of scl and mcl monomers, 3-hydroxybutyrate (3HB) and 3-hydroxydecanoate (3HD) was determined. The percentage of comonomer incorporation different to 3HB was 4.6 mol %. A glass transition temperature (T g ) of –50 oC and a melting temperature (T m ) of 79.8 oC were determined for the scl-mcl-PHA (3HB-co-3HDD). |
| Databáze: | OpenAIRE |
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