Establishing a community-wide DNA barcode library as a new tool for arctic research
| Autor: | Tomas Roslin, Riikka Kaartinen, Miroslav Barták, Paul D. N. Hebert, H. Disney, Mathias Jaschhof, Henry Väre, Søren Nielsen, Gergin Blagoev, L. Huldén, Christian Lettner, Jere Kahanpää, Stephanie Pedersen, Jari Ilmonen, Jaakko L. O. Pohjoismäki, Renee N Labbee, Tuomas Kankaanpää, Peter Gjelstrup, Jukka Salmela, Siegrun Ertl, Niels Martin Schmidt, T. R. Nielsen, Verner Michelsen, D. J. Gwiazdowicz, Helena Wirta, Lauri Paasivirta, Jevgeni Jakovlev, M. von Tschirnhaus, Claus Rasmussen, Pekka Vilkamaa, Paul Henning Krogh, Gergely Várkonyi |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Vascular plant Ribulose-Bisphosphate Carboxylase Greenland species Barcode 010603 evolutionary biology 01 natural sciences DNA barcoding law.invention diversity Electron Transport Complex IV 03 medical and health sciences Monophyly high arctic Abundance (ecology) law DNA Ribosomal Spacer arthropod Genetics Animals DNA Barcoding Taxonomic Ecology Evolution Behavior and Systematics Ecosystem Phylogeny biology Ecology Arctic Regions DNA barcode library Species diversity 15. Life on land Plants biology.organism_classification Biota 030104 developmental biology Taxon Arctic Biotechnology |
| Zdroj: | Wirta, H, Várkonyi, G, Rasmussen, C, Kaartinen, R, Schmidt, N M, Hebert, P, Barták, M, Blagoev, G, Disney, H, Ertl, S, Gjelstrup, P, Gwiazdowicz, D, Huldén, L, Ilmonen, J, Jakovlev, J, Jaschhof, M, Kahanpää, J, Kankaanpää, T, Krogh, P H, Labbee, R, Lettner, C, Michelsen, V, Nielsen, S A, Nielsen, T R, Paasivirta, L, Pedersen, S, Pohjoismäki, J, Salmela, J, Vilkamaa, P, Väre, H, von Tschirnhaus, M & Roslin, T 2016, ' Establishing a community-wide DNA barcode library as a new tool for arctic research ', Molecular Ecology Resources, vol. 16, no. 3, pp. 809-822 . https://doi.org/10.1111/1755-0998.12489 |
| DOI: | 10.1111/1755-0998.12489 |
| Popis: | DNA sequences offer powerful tools for describing the members and interactions of natural communities. In this study, we establish the to-date most comprehensive library of DNA barcodes for a terrestrial site, including all known macroscopic animals and vascular plants of an intensively studied area of the High Arctic, the Zackenberg Valley in Northeast Greenland. To demonstrate its utility, we apply the library to identify nearly 20 000 arthropod individuals from two Malaise traps, each operated for two summers. Drawing on this material, we estimate the coverage of previous morphology-based species inventories, derive a snapshot of faunal turnover in space and time and describe the abundance and phenology of species in the rapidly changing arctic environment. Overall, 403 terrestrial animal and 160 vascular plant species were recorded by morphology-based techniques. DNA barcodes (CO1) offered high resolution in discriminating among the local animal taxa, with 92% of morphologically distinguishable taxa assigned to unique Barcode Index Numbers (BINs) and 93% to monophyletic clusters. For vascular plants, resolution was lower, with 54% of species forming monophyletic clusters based on barcode regions rbcLa and ITS2. Malaise catches revealed 122 BINs not detected by previous sampling and DNA barcoding. The insect community was dominated by a few highly abundant taxa. Even closely related taxa differed in phenology, emphasizing the need for species-level resolution when describing ongoing shifts in arctic communities and ecosystems. The DNA barcode library now established for Zackenberg offers new scope for such explorations, and for the detailed dissection of interspecific interactions throughout the community. |
| Databáze: | OpenAIRE |
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