Engineering of Bacillus megaterium for improving PHA production from glycerol
| Autor: | I. de la Mata, Carlos del Cerro, Amanda Lucía Mora Martínez, Rodrigo Velasco-Bucheli, Javier Ricardo Gómez Cardozo |
|---|---|
| Přispěvatelé: | Colciencias (Colombia), Ministerio de Ambiente y Desarrollo Sostenible (Colombia), Gómez Cardozo, Javier Ricardo, Velasco-Bucheli, Rodrigo, Del Cerro, Carlos, De La Mata, I., Mora Martínez, Amanda Lucía, Gómez Cardozo, Javier Ricardo [0000-0001-5570-8194], Velasco-Bucheli, Rodrigo [0000-0002-1005-2475], Del Cerro, Carlos [0000-0003-1693-3895], De La Mata, I. [0000-0002-6637-2451], Mora Martínez, Amanda Lucía [0000-0002-3925-2278] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Glycerol 0303 health sciences biology Chemistry Glycerol dehydrogenase Polyhydroxyalkanoates fungi chemical and pharmacologic phenomena biology.organism_classification 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Bacillus megaterium Food science Molecular Biology 030304 developmental biology Biotechnology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 9 p.-4 fig. There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study, the improvement of glycerol uptake by a recombinant strain of B. megaterium carrying pHT01-bmgd was evaluated in order to enhance PHA production. The biomass and PHA production were evaluated and compared to wild-type. It was determined that the PHA produced by both strains was PHB and the highest improvement in PHB yield was 226% at 30 h. This study was funded by Departamento Administrativo de Ciencia, Tecnología e Innovación de Colombia (COLCIENCIAS) (Called 647 of 2014). The authors would like to thank COLCIENCIAS for funding. This work is framed at the “Contrato de acceso a recursos genéticos y productos derivados del Ministerio del Medio Ambiente y Desarrollo Sostenible de Colombia Nº95 and Nº159”. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|