Comparison of the ischemic and non-ischemic lung cancer metabolome reveals hyper activity of the TCA cycle and autophagy
| Autor: | Jiro Abe, Ryota Tanaka, Tomoyoshi Soga, Taku Sato, Hiroshi Shima, Yoshinori Okada, Miyuki Nomura, Nobuhiro Tanuma, Mami Morita, Yoshimi Sakamoto, Naohiko Kikuchi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lung Neoplasms Citric Acid Cycle Biophysics Ischemia Autophagy-Related Protein 7 Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Cell Line Tumor Autophagy medicine Metabolome Animals Lung cancer Molecular Biology Tumor metabolome business.industry Cancer Cell Biology Metabolism medicine.disease Metabolic pathway 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Female business Gene Deletion |
| Zdroj: | Biochemical and Biophysical Research Communications. 530:285-291 |
| ISSN: | 0006-291X |
| Popis: | Recent advances in cancer biology reveal the importance of metabolic changes in cancer; however, less is known about how metabolic pathways in tumors are regulated in vivo. Here, we report analysis of the lung cancer metabolism based on different surgical procedures, namely lobectomy and partial resection. In lobectomy, but not in partial resection, pulmonary arteries and veins are ligated prior to removal of tissues, rendering tissues ischemic. We show that tumors indeed undergo ischemia upon lobectomy and that the tumor metabolome differs markedly from that of tumors removed by partial resection. Comparison of the responses to ischemia in tumor and normal lung tissues revealed that lung cancer tissue exhibits greater TCA cycle and autophagic activity than do normal lung tissues in vivo in patients. Finally, we report that deleting ATG7, which encodes a protein essential for autophagy, antagonizes growth of tumors derived from lung cancer cell lines, suggesting that autophagy confers metabolic advantages to lung cancer. Our findings shed light on divergent metabolic responses to ischemia seen in tumors and normal tissues. |
| Databáze: | OpenAIRE |
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