Intranasal Dopamine Reduces In Vivo [(123)I]FP-CIT Binding to Striatal Dopamine Transporter: Correlation with Behavioral Changes and Evidence for Pavlovian Conditioned Dopamine Response
| Autor: | Maria A de Souza Silva, C. eMattern, C.I. eDecheva, Joseph P. Huston, A. eSadile, M. eBeu, H.W. eMüller, Susanne eNikolaus |
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| Přispěvatelé: | de Souza Silva, Maria A, Mattern, Claudia, Decheva, Cvetana, Huston, Joseph P, Sadile, Adolfo, Beu, Marku, Müller, H. W, Nikolaus, Susanne |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cognitive Neuroscience
Striatum Pharmacology Open field intranasal administration lcsh:RC321-571 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Neurochemical Dopamine Radioligand medicine lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry dopamine transporter Original Research Dopamine transporter biology Classical conditioning small animal SPECT 030227 psychiatry Neuropsychology and Physiological Psychology nervous system biology.protein placebo Nasal administration Pavlovian conditioning dopamine Psychology Neuroscience 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Frontiers in Behavioral Neuroscience Frontiers in Behavioral Neuroscience, Vol 10 (2016) |
| Popis: | Purpose: Dopamine (DA), which does not cross the blood-brain barrier, has central and behavioral effects when administered via the nasal route. Neither the mechanisms of central action of intranasal dopamine (IN-DA), nor its mechanisms of diffusion and transport into the brain are well understood. We here examined whether IN-DA application influences dopamine transporter (DAT) binding in the dorsal striatum and assessed the extent of binding in relation to motor and exploratory behaviors. We hypothesized that, based on the finding of increased extracellular DA in the striatum induced by application of IN-DA, binding of [123I]FP-CIT to the DAT should be decreased due to competition at the receptor.Methods: Rats were administered intranasal application of 3 mg/kg IN-DA and vehicle (VEH), with IN-DA injection either preceding or following VEH. Then motor and exploratory behaviors (traveled distance, velocity, center time, sitting, rearing, head-shoulder motility, grooming) were assessed for 30 min in an open field prior to administration of [123I]FP-CIT. DAT binding after IN-DA and VEH was measured with small animal SPECT two hours following administration of the radioligand. Results: 1) After IN-DA application, striatal DAT binding was significantly lower as compared to VEH, indicating that the nasally delivered dopamine had central action and increased DA levels comparable to that found previously with L-DOPA administration. 2) DAT binding in response to intranasal VEH was lower when IN-DA application preceded VEH treatment. This finding is suggestive of Pavlovian conditioning of DA at the level of the DAT, since the DA treatment modified (decreased) the binding in response to the subsequent VEH treatment. VEH treatment also reduced motor and exploratory behaviors more when applied before, as compared to when it followed IN-DA application, also indicative of behavioral Pavlovian conditioning akin to that found upon application of various psychostimulant drugs. Conclusions: The results (a) demonstrate a direct central action of intranasally applied dopamine on the dopamine transporter in the dorsal striatum, indicating enhanced DA availability and (b) provide first evidence of a Pavlovian conditioned DA response at the DA transporter. The latter results have relevance to understanding neurochemical mechanisms the underlie placebo action in the treatment of |
| Databáze: | OpenAIRE |
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