Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma- and precursor-assisted biomimetic process
| Autor: | Saori Miyata, Yukimi Kanemoto, Yuto Yoshino, Ayako Oyane, Arputharaj Joseph Nathanael, Tsutomu Sugaya, Hirofumi Miyaji, Erika Nishida, Kayoko Mayumi, Tsukasa Akasaka, Akihito Kato, Syama Santhakumar |
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| Rok vydání: | 2021 |
| Předmět: |
Scaffold
Bone Regeneration osteogenic differentiation Apatite bone augmentation Dogs In vivo Biomimetics Osteogenesis Apatites medicine Animals Dental alveolus Tissue Engineering Tissue Scaffolds Chemistry Furcation defect cyto-compatibility Rats periodontal healing Cell culture visual_art immunohistochemistry Collagenase visual_art.visual_art_medium Periodontics dog class II furcation defect model Collagen low-crystalline apatite Biomedical engineering Protein adsorption medicine.drug |
| Zdroj: | Journal of periodontal researchREFERENCES. 57(1) |
| ISSN: | 1600-0765 |
| Popis: | Background and objectives In the treatment of severe periodontal destruction, there is a strong demand for advanced scaffolds that can regenerate periodontal tissues with adequate quality and quantity. Recently, we developed a plasma- and precursor-assisted biomimetic process by which a porous collagen scaffold (CS) could be coated with low-crystalline apatite. The apatite-coated collagen scaffold (Ap-CS) promotes cellular ingrowth within the scaffold compared to CS in rat subcutaneous tissue. In the present study, the osteogenic activity of Ap-CS was characterized by cell culture and rat skull augmentation tests. In addition, the periodontal tissue reconstruction with Ap-CS in a beagle dog was compared to that with CS. Methods The plasma- and precursor-assisted biomimetic process was applied to CS to obtain Ap-CS with a low-crystalline apatite coating. The effects of apatite coating on the scaffold characteristics (i.e., surface morphology, water absorption, Ca release, protein adsorption, and enzymatic degradation resistance) were assessed. Cyto-compatibility and the osteogenic properties of Ap-CS and CS were assessed in vitro using preosteoblastic MC3T3-E1 cells. In addition, we performed in vivo studies to evaluate bone augmentation and periodontal tissue reconstruction with Ap-CS and CS in a rat skull and canine furcation lesion, respectively. Results As previously reported, the plasma- and precursor-assisted biomimetic process generated a low-crystalline apatite layer with a nanoporous structure that uniformly covered the Ap-CS surface. Ap-CS showed significantly higher water absorption, Ca release, lysozyme adsorption, and collagenase resistance than CS. Cell culture experiments revealed that Ap-CS was superior to CS in promoting the osteoblastic differentiation of MC3T3-E1 cells while suppressing their proliferation. Additionally, Ap-CS significantly promoted (compared to CS) the augmentation of the rat skull bone and showed the potential to regenerate alveolar bone in a dog furcation defect. Conclusion Ap-CS fabricated by the plasma- and precursor-assisted biomimetic process provided superior promotion of osteogenic differentiation and bone neoformation compared to CS. |
| Databáze: | OpenAIRE |
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