White Adipose Tissue Surface Expression of LDLR and CD36 is Associated with Risk Factors for Type 2 Diabetes in Adults with Obesity

Autor: Yannick Cyr, Emmanuelle Loizon, Gaétan Mayer, Valérie Lamantia, Emilienne Tudor Ngo Sock, Simon Bissonnette, Hubert Vidal, Michel Chrétien, Hanny Wassef, May Faraj
Přispěvatelé: Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Canadian Institutes of Health Research (CIHR)13359893581123409
Rok vydání: 2020
Předmět:
CD36 Antigens
Male
medicine.medical_specialty
Diabetes risk
Apolipoprotein B
Adipose Tissue
White
Endocrinology
Diabetes and Metabolism
CD36
Medicine (miscellaneous)
Adipose tissue
030209 endocrinology & metabolism
White adipose tissue
03 medical and health sciences
0302 clinical medicine
Endocrinology
Insulin resistance
Risk Factors
Internal medicine
medicine
Humans
Obesity
030212 general & internal medicine
Aged
Nutrition and Dietetics
biology
business.industry
food and beverages
nutritional and metabolic diseases
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Middle Aged
Postprandial Period
medicine.disease
Cross-Sectional Studies
Postprandial
Diabetes Mellitus
Type 2
Receptors
LDL
LDL receptor
biology.protein
Female
lipids (amino acids
peptides
and proteins)
business
hormones
hormone substitutes
and hormone antagonists
Zdroj: Obesity
Obesity, Wiley, 2020, 28 (12), pp.2357-2367. ⟨10.1002/oby.22985⟩
ISSN: 1930-739X
1930-7381
Popis: International audience; Objective Human conditions with upregulated receptor uptake of low-density lipoproteins (LDL) are associated with diabetes risk, the reasons for which remain unexplored. LDL induce metabolic dysfunction in murine adipocytes. Thus, it was hypothesized that white adipose tissue (WAT) surface expression of LDL receptor (LDLR) and/or CD36 is associated with WAT and systemic metabolic dysfunction. Whether WAT LDLR and CD36 expression is predicted by plasma lipoprotein-related parameters was also explored. Methods This was a cross-sectional analysis of 31 nondiabetic adults (BMI > 25 kg/m(2)) assessed for WAT surface expression of LDLR and CD36 (immunohistochemistry), WAT function, WAT and systemic inflammation, postprandial fat metabolism, and insulin resistance (IR; hyperinsulinemic-euglycemic clamp). Results Fasting WAT surface expression of LDLR and CD36 was negatively associated with WAT function (H-3-triglyceride storage,r = -0.45 and -0.66, respectively) and positively associated with plasma IL-1 receptor antagonist (r = 0.64 and 0.43, respectively). Their expression was suppressed 4 hours postprandially, and reduced LDLR was further associated with IR (M/I-clamp,r = 0.61 women,r = 0.80 men). Plasma apolipoprotein B (apoB)-to-PCSK9 ratio predicted WAT surface expression of LDLR and CD36, WAT dysfunction, WAT NLRP3 inflammasome priming and disrupted cholesterol-sensing genes, and systemic IR independent of sex and body composition. Conclusions Higher fasting and lower postprandial WAT surface expression of LDLR and CD36 is associated with WAT dysfunction, systemic inflammation, and IR in adults with overweight/obesity, anomalies that are predicted by higher plasma apoB-to-PCSK9 ratio.
Databáze: OpenAIRE
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