Simultaneous measurement of dolasetron and its major metabolite, MDL 74,156, in human plasma and urine
| Autor: | K.D. Haegele, J.-J. Schwartz, N.D. Huebert, L. Zeidler, V. Schwach |
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| Přispěvatelé: | Marion Merrell Dow, Strasbourg, France, Institut de chimie et procédés pour l'énergie, l'environnement et la santé (ICPEES), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Schwartz, Jean-Jacques |
| Rok vydání: | 1996 |
| Předmět: |
Indoles
Time Factors Calibration curve Metabolite MESH: Quinolizines Urine 030226 pharmacology & pharmacy 01 natural sciences High-performance liquid chromatography MESH: Serotonin Antagonists MESH: Linear Models 03 medical and health sciences chemistry.chemical_compound MESH: Osmolar Concentration 0302 clinical medicine Blood plasma Humans Fluorometry MESH: Circadian Rhythm MESH: Chromatography High Pressure Liquid MESH: Alcohols Chromatography High Pressure Liquid MESH: Indoles Reproducibility MESH: Humans Chromatography Chemistry MESH: Time Factors 010401 analytical chemistry Osmolar Concentration MESH: Fluorometry Reproducibility of Results General Chemistry Reversed-phase chromatography [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences MESH: Injections Intravenous 0104 chemical sciences Circadian Rhythm [SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences MESH: Reproducibility of Results Alcohols Injections Intravenous Linear Models Serotonin Antagonists Quantitative analysis (chemistry) Quinolizines |
| Zdroj: | J Chromatogr B Biomed Appl J Chromatogr B Biomed Appl, 1996, 685 (2), pp.291-7 |
| ISSN: | 1572-6495 |
| Popis: | International audience; A selective and sensitive analytical method for the simultaneous measurement of dolasetron (I) and its major metabolite, MDL 74,156 (II), in human plasma and urine samples has been developed using a structural analogue. MDL 101,858, as internal standard (I.S.). The compounds were extracted from plasma and urine using solvent extraction after the addition of the I.S. Chromatographic separation was carried out on a reversed-phase HPLC column and detection and quantification was by fluorescence with excitation and emission wavelengths of 285 and 345 nm, respectively. Linear responses were obtained over concentration ranges of 5 to 1000 pmol/ml for plasma samples and 20 to 1000 pmol/ml for urine samples with correlation coefficients for the calibration curves exceeding 0.999 in all cases. Intra-day and inter-day reproducibility yielded limits of quantification of 10 pmol/ml for I and 5 pmol/ml for II plasma and 50 pmol/ml for I and II in urine. The method has been applied to the simultaneous analysis of both compounds in plasma and urine samples coming from clinical pharmacokinetic studies. |
| Databáze: | OpenAIRE |
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