Identification and characterization of high glucose and glucosamine responsive element in the rat osteopontin promoter
| Autor: | Ayako Take, Koutaro Yokote, Seijiro Mori, Amy L. Ridall, Kazuki Kobayashi, Sunao Asaumi, Harukiyo Kawamura, Minoru Takemoto, Masaki Fujimoto, William T. Butler, Yasushi Saito |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Transcription Genetic Sialoglycoproteins Endocrinology Diabetes and Metabolism Gene Expression E-box Response Elements Transfection Muscle Smooth Vascular Mice chemistry.chemical_compound Endocrinology stomatognathic system Transcription (biology) Glucosamine Gene expression Internal Medicine Animals Humans Luciferase Osteopontin Rats Wistar Promoter Regions Genetic Aorta Cells Cultured Conserved Sequence Expression vector Base Sequence biology Promoter Molecular biology Rats Glucose Biochemistry chemistry Mutagenesis biology.protein Gene Deletion |
| Zdroj: | Journal of Diabetes and its Complications. 17:34-38 |
| ISSN: | 1056-8727 |
| DOI: | 10.1016/s1056-8727(02)00189-7 |
| Popis: | We have previously reported that high glucose stimulates osteopontin (OPN) expression via a protein kinase C-dependent pathway and a hexosamine pathway in cultured rat aortic smooth muscle cells (SMCs) [Biochem. Biophys. Res. Commun. 258 (1999) 722.]. In the present study, we carried out functional OPN promoter assays using the luciferase expression vector system in cultured rat aortic SMCs to determine a high glucose/glucosamine responsive element. An extensive deletion analysis of the 5′-flanking region of the rat OPN gene revealed that an element involved in high glucose and glucosamine responses was present within a region between −112 and −62 bp of the OPN promoter. This region is highly conserved in the rat, mouse, and human promoters and contains a number of consensus regions, including an E-box and a GC-rich region. Mutation of the E-box or the GC-rich region resulted in a significant loss of both high glucose and glucosamine responses. These results suggest that two cis-acting elements, the E-box and the GC-rich region, are involved at least partly in high glucose/glucosamine-stimulated transcription of the rat OPN gene. |
| Databáze: | OpenAIRE |
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