Overexpressed Genes/ESTs and Characterization of Distinct Amplicons on 17823 in Breast Cancer Cells
| Autor: | Bethany L. Niell, Elizabeth M. Petty, Samir M. Hanash, Jean Marie Rouillard, Ayse E. Erson, Scott K. DeMers |
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| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Biology lcsh:RC254-282 law.invention 03 medical and health sciences breast cancer 0302 clinical medicine law Gene duplication Northern blot Gene Polymerase chain reaction MUL 030304 developmental biology Southern blot Genetics 0303 health sciences Expressed sequence tag Oncogene TBX2 Amplicon lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Molecular biology 3. Good health 17823 amplification RPS6KB1 030220 oncology & carcinogenesis |
| Zdroj: | Neoplasia: An International Journal for Oncology Research, Vol 3, Iss 6, Pp 521-526 (2001) |
| ISSN: | 1476-5586 |
| DOI: | 10.1038/sj.neo.7900187 |
| Popis: | 17823 is a frequent site of gene amplification in breast cancer. Several lines of evidence suggest the presence of multiple amplicons on 17823. To characterize distinct amplicons on 17823 and localize putative oncogenes, we screened genes and expressed sequence tags (ESTs) in existing physical and radiation hybrid maps for amplification and overexpression in breast cancer cell lines by semiquantitative duplex PCR, semiquantitative duplex RT-PCR, Southern blot, Northern blot analyses. We identified two distinct amplicons on 17823, one including TBX2 and another proximal region including RPS6KB1 (PS6K) and MUL. In addition to these previously reported overexpressed genes, we also identified amplification and overexpression of additional uncharacterized genes and ESTs, some of which suggest potential oncogenic activity. In conclusion, we have further defined two distinct regions of gene amplification and overexpression on 17823 with identification of new potential oncogene candidates. Based on the amplification and overexpression patterns of known and as of yet unrecognized genes on 17823, it is likely that some of these genes mapping to the discrete amplicons function as oncogenes and contribute to tumor progression in breast cancer cells. |
| Databáze: | OpenAIRE |
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