Pterygial body epithelium domination of pterygial proliferation with TCF4 as a potential key factor
| Autor: | Siying Xu, Cong Nie, Rong Lu, Yadan Quan, Zhixin Tang, Xinchun Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Abcg2 proliferation 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation lcsh:Ophthalmology Medicine pterygium Transcription factor biology business.industry TCF4 medicine.disease Epithelium Proliferating cell nuclear antigen Pterygium Staining Ophthalmology 030104 developmental biology medicine.anatomical_structure Basic Research lcsh:RE1-994 030221 ophthalmology & optometry biology.protein business epithelium transcription factor 4 |
| Zdroj: | International Journal of Ophthalmology, Vol 11, Iss 9, Pp 1467-1474 (2018) |
| Popis: | AIM: To characterize the proliferative capacity of pterygial epithelium in different regions (head, neck and body) of pterygium and explore the function of transcription factor 4 (TCF4) in pterygium proliferation. METHODS: Thirty pterygium tissues and 10 normal conjunctival tissues were obtained from Zhongshan Ophthalmic Center (ZOC) and Guangdong Eye Bank, respectively. Proliferative capacity of head, neck and body in pterygial epithelium was measured using clonal analysis, fold growth analysis and expression profile of proliferative markers revealed by immunofluorescent staining and real-time PCR. The expression of TCF4 was highlighted by double immunofluorescent staining with other proliferation related markers such as proliferating cell nuclear antigen (PCNA) and ATP-binding cassette sub-family G member 2 (ABCG2). RESULTS: The proliferative potential of pterygial epithelium was higher than that of normal conjunctival epithelium. High expression levels of proliferative markers (P63α, PCNA and ABCG2) in pterygial body epithelium were observed in immunofluorescent staining and real-time PCR (P |
| Databáze: | OpenAIRE |
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