Functional genomics of genes with small open reading frames (sORFs) in S. cerevisiae
| Autor: | Wei Chun Au, Li Ni, Ronald W. Davis, Munira A. Basrai, David C. Wheeler, Carole D. Carter, Hui Yang, Michael Snyder, Jef D. Boeke, Lauren E. Kitchen, Angela Chu, James P. Kastenmayer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae
Haploidy Genome Evolution Molecular Open Reading Frames Genetics Humans Letters ORFS Gene Conserved Sequence Genetics (clinical) biology Genome Human Gene Expression Profiling biology.organism_classification Open reading frame Phenotype Gene Expression Regulation Eukaryote Genome Fungal Functional genomics Gene Deletion DNA Damage Genetic screen |
| Popis: | Genes with small open reading frames (sORFs; Saccharomyces cerevisiae. Based on independent experimental approaches and computational analyses, evidence exists for 299 sORFs in the S. cerevisiae genome, representing ∼5% of the annotated ORFs. We determined that a similar percentage of sORFs are annotated in other eukaryotes, including humans, and 184 of the S. cerevisiae sORFs exhibit similarity with ORFs in other organisms. To investigate sORF function, we constructed a collection of gene-deletion mutants of 140 newly identified sORFs, each of which contains a strain-specific “molecular barcode,” bringing the total number of sORF deletion strains to 247. Phenotypic analyses of the new gene-deletion strains identified 22 sORFs required for haploid growth, growth at high temperature, growth in the presence of a nonfermentable carbon source, or growth in the presence of DNA damage and replication-arrest agents. We provide a collection of sORF deletion strains that can be integrated into the existing deletion collection as a resource for the yeast community for elucidating gene function. Moreover, our analyses of the S. cerevisiae sORFs establish that sORFs are conserved across eukaryotes and have important biological functions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|