Characterization of amniotic stem cells
| Autor: | Moustafa Fathy, Takeda Yuji, Toshio Nikaido, Chika Koike, Toshiko Yoshida, Motonori Okabe, Kaixuan Zhou, Yukio Kato, Yukio Nakamura |
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| Rok vydání: | 2014 |
| Předmět: |
Stem Cells
Amniotic stem cells Cell Biology Cell Separation Original Articles Biology Stem cell marker Molecular biology Antigens Differentiation Kruppel-Like Factor 4 Gene Expression Regulation Amniotic epithelial cells Neurosphere embryonic structures Humans CD90 Female Amnion Stem cell reproductive and urinary physiology Cells Cultured Developmental Biology Biotechnology Stem cell transplantation for articular cartilage repair Adult stem cell |
| Zdroj: | Cellular reprogramming. 16(4) |
| ISSN: | 2152-4998 |
| Popis: | The amnion membrane is developed from embryo-derived cells, and amniotic cells have been shown to exhibit multidifferentiation potential. These cells represent a desirable source for stem cells for a variety of reasons. However, to date very few molecular analyses of amnion-derived cells have been reported, and efficient markers for isolating the stem cells remain unclear. This paper assesses the characterization of amnion-derived cells as stem cells by examining stemness marker expressions for amnion-derived epithelial cells and mesenchymal cells by flow cytometry, immunocytochemistry, and quantitative PCR. Flow cytometry revealed that amnion epithelial cells expressed CD133, CD 271, and TRA-1-60, whereas mecenchymal cells expressed CD44, CD73, CD90, and CD105. Immunohistochemistry showed that both cells expressed the stemness markers Oct3/4, Sox2, Klf4, and SSEA4. Stemness genes' expression in amnion epithelial cells, mesenchymal cells, fibroblast, bone marrow–derived mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSCs) was compared by quantitative reverse-transcription polymerase chain reaction (RT-PCR). Amnion-derived epithelial cells and mesenchymal cells expressed Oct3/4, Nanog, and Klf4 more than bone marrow–derived MSCs. The sorted TRA1-60–positive cells expressed Oct3/4, Nanog, and Klf4 more than unsorted cells or TRA1-60–negative cells. TRA1-60 can be a marker for isolating amnion epithelial stem cells. |
| Databáze: | OpenAIRE |
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