Genomic RNA of mengovirus. VI. Translation of its two cistrons in lysates of interferon-treated cells
| Autor: | R Pérez-Bercoff, J Facchini, A. M. Degener, P Pagnotti |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Immunology
Biology Microbiology Ribosome Mice Viral Proteins chemistry.chemical_compound L Cells Virology Mengovirus Protein biosynthesis Animals RNA Messenger Peptide Chain Initiation Translational Nuclease Methionine RNA Translation (biology) biology.organism_classification Molecular biology Poly I-C chemistry Biochemistry Protein Biosynthesis Insect Science biology.protein RNA Viral Interferons Eukaryotic Ribosome Ribosomes Research Article |
| Zdroj: | Journal of Virology. 45:889-894 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.45.2.889-894.1983 |
| Popis: | The addition of low levels (40 ng/ml) of the synthetic double-stranded polyribonucleotide poly I:C to lysates of interferon-treated L-cells resulted in a strong inhibition (70 to 75%) of the in vitro translation of mengovirus RNA. Under these conditions, the rates of incorporation of [35S]methionine or formyl-[35S]methionine were depressed to a comparable extent. The sequences of mengovirus RNA recognized by ribosomes of interferon-treated cells at initiation of translation were compared with those present in initiation complexes formed by ribosomes of untreated controls. Fingerprint analysis revealed that the same sequences of mengovirus RNA were protected against nuclease attack by the 80S and the 40S initiation complexes formed in vitro in lysates of control or interferon-treated L-cells. Mengovirus RNA-coded proteins were labeled at their N-terminal end with formyl-[35S]methionine and digested to completion with trypsin. The resulting fragments were separated by high-voltage paper electrophoresis. Two different formyl-[35S]methionine-labeled N termini were resolved. Further analyses supported the notion that the two radioactive peaks originated in the initiation of translation at two different sites. This pattern did not change when mengovirus RNA was translated in lysates of interferon-treated cells. |
| Databáze: | OpenAIRE |
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