Tandem duplications of the lac region of the Escherichia coli chromosome
| Autor: | J D Heath, George M. Weinstock |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Unequal crossing over
Myoviridae Biology medicine.disease_cause Biochemistry Bacteriophage Transduction Genetic Gene duplication Escherichia coli medicine Promoter Regions Genetic Repetitive Sequences Nucleic Acid Recombination Genetic Gel electrophoresis Genetics General Medicine Chromosomes Bacterial biology.organism_classification Molecular biology Enterobacteriaceae Lac Operon Genes Bacterial Multigene Family Recombination Plasmids |
| Zdroj: | Biochimie. 73:343-352 |
| ISSN: | 0300-9084 |
| Popis: | Tandem duplications are caused by unequal crossing over between homologous sequences. Duplications in the lac region of the Escherichia coli chromosome were isolated by two methods. Duplication frequency using a method involving P1 transduction increased from 0.4% with no UV to 2.0% following UV irradiation at 35 J/m2. Duplication frequency in lac using a second generalizable method that does not involve P1 transduction increased from 0.7 to 12% at 35 J/m2 UV. In both cases the duplication frequency began to plateau at UV doses of 12 J/m2 and 24 J/m2. According to segregation analysis of sixteen duplications there may be at least seven classes of duplications isolated by each method. Pulsed-field gel electrophoresis was used to measure the duplications isolated without P1 transduction. The minimum size of the duplications ranged from 30 to 320 kb but could be much larger. |
| Databáze: | OpenAIRE |
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