Freeze-drying of monoclonal antibody-conjugated gold nanorods: Colloidal stability and biological activity
| Autor: | Samer R. Abulateefeh, Khaled M. Al-Qaoud, Alaaldin M. Alkilany, Majd A. Hamaly |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Lymphoma
B-Cell Cryoprotectant Pharmaceutical Science Nanoparticle 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Colloid Freeze-drying chemistry.chemical_compound Cryoprotective Agents Drug Stability medicine Humans Mannitol Colloids Nanotubes Chemistry digestive oral and skin physiology Trehalose 021001 nanoscience & nanotechnology Antigens CD20 0104 chemical sciences Freeze Drying Colloidal gold Biophysics Gold 0210 nano-technology Rituximab medicine.drug |
| Zdroj: | International journal of pharmaceutics. 550(1-2) |
| ISSN: | 1873-3476 |
| Popis: | Maintaining colloidal stability of nanoparticles in suspensions is a major challenge. Therefore, freeze-drying (lyophilization) is recently proposed to preserve colloidal stability of nanoparticles through maintaining them in a solid state. However, freeze-drying would itself induce nanoparticle aggregation unless proper formulation with a careful selection of cryoprotectants is considered. Herein, we evaluate the colloidal stability of gold nanorods (GNRs) conjugated with a rituximab as a model monoclonal antibody upon freeze-drying in the presence of various cryoprotectants (mannitol, trehalose and sucrose). Aggregation-induced optical responses of GNRs were used as a sensitive tool to follow nanoparticle aggregation. In the absence of cryoprotectants, rituximab-conjugated GNRs aggregate irreversibly while evaluated cryoprotectants exhibit a significant protective effect. Maximal colloidal stability of GNRs is observed in the presence of trehalose while mannitol results in best cake formation in terms of shape and integrity. A combination of trehalose and mannitol produces a lyophilized product with satisfactory GNR colloidal stability and cake shape. Moreover, we show that freeze-dried rituximab-conjugated GNRs in presence of proper cryoprotectants maintain typical binding to lymphoma tissues as confirmed via immunohistochemistry assay. |
| Databáze: | OpenAIRE |
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