Plasmid DNA Topology Assayed by Two-Dimensional Agarose Gel Electrophoresis

Autor:	María Luisa Martínez-Robles, Dora B. Krimer, Jorge Bernardo Schvartzman, Pablo E. Hernández
Přispěvatelé:	Ministerio de Economía y Competitividad (España), Schvartzman, Jorge Bernardo, Hernandez, Pablo, Krimer, Dora B., Schvartzman, Jorge Bernardo [0000-0002-1856-6278], Hernandez, Pablo [0000-0002-8113-1277], Krimer, Dora B. [0000-0001-9972-3994]
Rok vydání:	2013
Předmět:	Knots Plasmid preparation Gel electrophoresis Gel electrophoresis of nucleic acids Supercoiling Chemistry Topoisomerases Catenanes DNA replication Topology DNA topology chemistry.chemical_compound Molecular-weight size marker Agarose gel electrophoresis DNA supercoil DNA
Zdroj:	Methods in Molecular Biology ISBN: 9781627035644 Digital.CSIC. Repositorio Institucional del CSIC instname
DOI:	10.1007/978-1-62703-565-1_7
Popis:	12 p.-3 fig. Two-dimensional (2D) agarose gel electrophoresis is nowadays one of the best methods available to analyze DNA molecules with different masses and shapes. The possibility to use nicking enzymes and intercalating agents to change the twist of DNA during only one or in both runs, improves the capacity of 2D gels to discern molecules that apparently may look alike. Here we present protocols where 2D gels are used to understand the structure of DNA molecules and its dynamics in living cells. This knowledge is essential to comprehend how DNA topology affects and is affected by all the essential functions that DNA is involved in: replication, transcription, repair and recombination. This work was sustained by grant BFU2011-22489 to J.B.S. from the Spanish Ministerio de Economía y Competitividad.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6594655af3d255a957c5f8f3ae0a3c8d https://doi.org/10.1007/978-1-62703-565-1_7 Zobrazit plný text záznamu