Transcriptome analysis of Plasmodium falciparum isolates from Benin reveals specific gene expression associated with cerebral malaria
| Autor: | E, Guillochon, J, Fraering, V, Joste, C, Kamaliddin, B, Vianou, L, Houzé, L G, Baudrin, J F, Faucher, A, Aubouy, S, Houzé, M, Cot, N, Argy, O, Taboureau, G I, Bertin, Brigitte, Techer |
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| Přispěvatelé: | Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 261), Institut de Recherche pour le Développement (IRD)-Université Paris Cité (UPCité), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), University of Calgary, Institut de Recherche Clinique du Bénin [Abomey-Calavi, Bénin] (IRCB), Institut Curie [Paris], Université Paris sciences et lettres (PSL), Epidémiologie des Maladies Chroniques en zone tropicale (EpiMaCT), CHU Limoges-Institut d'Epidémiologie Neurologique et de Neurologie Tropicale-Institut National de la Santé et de la Recherche Médicale (INSERM)-OmégaHealth (ΩHealth), Université de Limoges (UNILIM)-Université de Limoges (UNILIM), Service des Maladies infectieuses et tropicales [CHU Limoges], CHU Limoges, Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), ANR-17-CE17-0001,NEUROCM,Identification des facteurs parasitaires et de l'hôte à l'origine de la neuroinflammation et de sa résolution dans un contexte de neuropaludisme(2017) |
| Rok vydání: | 2021 |
| Předmět: |
Erythrocytes
Plasmodium falciparum Malaria Cerebral Protozoan Proteins Biology Microbiology Transcriptome transcriptomics var genes Antigen parasitic diseases Gene expression Benin Humans Immunology and Allergy Malaria Falciparum Child Receptor Gene Gene Expression Profiling biology.organism_classification Entry into host Infectious Diseases Cerebral Malaria Child Preschool cytoadherence cerebral malaria [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie RNA-seq |
| Zdroj: | Journal of Infectious Diseases Journal of Infectious Diseases, 2022, 225 (12), ⟨10.1093/infdis/jiac086⟩ |
| ISSN: | 0022-1899 1537-6613 |
| DOI: | 10.1101/2021.11.08.467248 |
| Popis: | The host and parasitic factors leading to cerebral malaria (CM) are not yet fully elucidated and CM Plasmodium falciparum isolates transcriptome profile remains largely unknown. Based on RNA-seq data from 15 CM and 15 uncomplicated malaria (UM) children from Benin, we identified an increased ring stage signature in CM parasites. Reduced circulating time may result from a higher adherence ability of CM isolates and consistent with this hypothesis, we measured an overexpression of var genes in CM. var genes domains expression was more restricted in CM isolates compared to UM, reflecting the specific binding to receptors in host brain endothelium capillaries. However, ICAM-1 binding motif was found expressed in both CM and UM, questioning its role in PfEMP1 adhesion to ICAM-1 receptor. UM isolates increased circulation time may also be modulated by a more efficient immune response against infected erythrocytes surface proteins, which we could not demonstrate on our cohort. Identification of deregulated genes involved in adhesion, excluding variant surface antigens, also supports the hypothesis of an increased CM adhesion capacity. Finally, numerous upregulated genes involved in entry into host pathway were found, reflecting a greater erythrocytes invasion capacity of CM parasites. |
| Databáze: | OpenAIRE |
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