GPRC5A facilitates cell proliferation through cell cycle regulation and correlates with bone metastasis in prostate cancer
| Autor: | Yuta Yanagihara, Balázs Győrffy, Hiroyuki Iio, Shuhei Yoshida, Yohei Miyagi, Takeshi Kishida, Noritaka Saeki, Aoi Ikedo, Takashi Saika, Iori Sakakibara, Tadahiko Kikugawa, Yoshiyasu Nakamura, Yoichiro Okubo, Yuuki Imai, Yuichiro Sawada |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Cancer Research Cell cycle checkpoint Cell Mice Nude Bone Neoplasms Receptors G-Protein-Coupled Gene Knockout Techniques Mice 03 medical and health sciences Prostate cancer 0302 clinical medicine Prostate Animals Humans Medicine Phosphorylation Cyclic AMP Response Element-Binding Protein Cell Proliferation Mice Inbred BALB C business.industry Cell growth Gene Expression Profiling Prostatic Neoplasms GPRC5A Bone metastasis Cell Cycle Checkpoints Cell cycle medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis PC-3 Cells Cancer research Heterografts business |
| Zdroj: | International Journal of Cancer. 146:1369-1382 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.32554 |
| Popis: | The prognosis of patients with progressive prostate cancers that are hormone refractory and/or have bone metastasis is poor. Multiple therapeutic targets to improve prostate cancer patient survival have been investigated, including orphan GPCRs. In our study, we identified G Protein-Coupled Receptor Class C Group 5 Member A (GPRC5A) as a candidate therapeutic molecule using integrative gene expression analyses of registered data sets for prostate cancer cell lines. Kaplan-Meier analysis of TCGA data sets revealed that patients who have high GPRC5A expression had significantly shorter overall survival. PC3 prostate cancer cells with CRISPR/Cas9-mediated GPRC5A knockout exhibited significantly reduced cell proliferation both in vitro and in vivo. RNA-seq revealed that GPRC5A KO PC3 cells had dysregulated expression of cell cycle-related genes, leading to cell cycle arrest at the G2/M phase. Furthermore, the registered gene expression profile data set showed that the expression level of GPRC5A in original lesions of prostate cancer patients with bone metastasis was higher than that without bone metastasis. In fact, GPRC5A KO PC3 cells failed to establish bone metastasis in xenograft mice models. In addition, our clinical study revealed that GPRC5A expression levels in prostate cancer patient samples were significantly correlated with bone metastasis as well as the patient's Gleason score (GS). Combined assessment with the immunoreactivity of GPRC5A and GS displayed higher specificity for predicting the occurrence of bone metastasis. Together, our findings indicate that GPRC5A can be a possible therapeutic target and prognostic marker molecule for progressive prostate cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text