Histamine prevents functional and morphological alterations of submandibular glands induced by ionising radiation
| Autor: | Máximo Croci, Juan Pablo Prestifilippo, Elena Rivera, Eliana Carabajal, Vanina Araceli Medina, Rosa Bergoc, Juan C. Elverdin |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Submandibular Gland Apoptosis Biology Xerostomia Salivary Glands Ionizing radiation Rats Sprague-Dawley chemistry.chemical_compound Radiation Ionizing Internal medicine medicine Animals Radiology Nuclear Medicine and imaging Radiation Injuries Saline Radiotherapy Radiological and Ultrasound Technology Salivary gland Cancer medicine.disease Submandibular gland Rats Radiation therapy medicine.anatomical_structure Endocrinology chemistry Head and Neck Neoplasms Histamine |
| Zdroj: | International Journal of Radiation Biology. 87:284-292 |
| ISSN: | 1362-3095 0955-3002 |
| Popis: | Xerostomia is a common, disturbing side-effect among patients treated with radiotherapy for head-and-neck cancer. The aim of the present work was to investigate whether histamine could prevent salivary gland dysfunction and histological alterations exerted by ionising radiation.Forty-eight rats were divided into four groups. Histamine and histamine-5 Gy groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 24 h before irradiation. Histamine-5 Gy and untreated-5 Gy groups were irradiated with a single dose of whole-body Cesium-137 irradiation. Control and untreated-5 Gy groups were given daily saline injections. Three days post irradiation metacholine-induced salivary secretion was measured or animals were sacrificed and submandibular gland (SMG) removed, stained and histological characteristics were evaluated. Proliferation and apoptosis markers were studied by immunohistochemistry.Radiation decreased salivary secretion by 40% in comparison to untreated rats, which was associated with loss of SMG mass, alteration of epithelial architecture, partial loss of secretor granular material, diminished proliferation and a remarkable apoptotic response. In contrast, histamine completely reversed the reduced salivation induced by radiation, conserved glandular mass with normal appearance and preserved the structural organisation of secretor granules. Radiation-induced toxicity is prevented by histamine essentially by suppressing apoptosis of ductal and acinar cells, reducing the number of apoptotic cells per field (19.0 ± 3.8 vs. 106.0 ± 12.0 in untreated animals, P0.001), and also by preventing the radiation-induced decrease in cell proliferation.Histamine prevents morphological and functional radiation-induced damage on SMG, representing a potential radioprotector for treatment of patients undergoing radiotherapy for head and neck malignancies. |
| Databáze: | OpenAIRE |
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