Ion-sensitive microelectrode system with short response time
| Autor: | Sören Schreiber, Peter Scheid, A. De Santis, Y. Okada, K. Muckenhoff |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Ions
Neurons Membrane potential Frequency response Time Factors business.industry Chemistry General Neuroscience Analytical chemistry Response time Hydrogen-Ion Concentration Membrane Potentials Ion selective electrode Ion Electrophysiology Microelectrode Linearization Electrode Optoelectronics business Microelectrodes |
| Zdroj: | Journal of Neuroscience Methods. 51:147-153 |
| ISSN: | 0165-0270 |
| Popis: | The measurement of changes in ion activity (e.g., pH) in neurons requires fine tip-sized double-barreled microelectrodes: one channel being equipped with an ion-selective liquid membrane, the other used for measurement of the membrane potential. The limited bandwidth and the differing transfer functions for electrical and ionic signals necessitate frequency response linearization networks to ensure that the output signal of the electrode is a faithful image of the input signal. We have developed a linearization network to ensure a rapid response time for ion-sensitive microelectrodes. To test the response characteristic we have developed a test system that allows the pH at the electrode tip to be changed within 1 ms. Application of these techniques to electrodes of 1 micron tip diameter results in a 90% response time to a pH step of approximately 60 ms and of approximately 2 ms with electrodes with 20 micron tip. |
| Databáze: | OpenAIRE |
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