A Robust Auxin Response Network Controls Embryo and Suspensor Development through a Basic Helix Loop Helix Transcriptional Module
| Autor: | Jos R. Wendrich, Mark V. Boekschoten, Che-Yang Liao, Lieke Vlaar, Raju Datla, Cristina I. Llavata-Peris, Guido J. E. J. Hooiveld, Daoquan Xiang, Annemarie S. Lokerse, Dolf Weijers, Tatyana Radoeva |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Cell fate determination 01 natural sciences In Brief Transcriptome 03 medical and health sciences Auxin Gene Expression Regulation Plant Arabidopsis chemistry.chemical_classification biology Auxin homeostasis Indoleacetic Acids Arabidopsis Proteins fungi food and beverages Embryo Cell Biology biology.organism_classification Plants Genetically Modified Cell biology 030104 developmental biology chemistry embryonic structures Seeds Reprogramming Suspensor 010606 plant biology & botany Signal Transduction |
| Zdroj: | The Plant cell. 31(1) |
| ISSN: | 1532-298X |
| Popis: | Land plants reproduce sexually by developing an embryo from a fertilized egg cell. However, embryos can also be formed from other cell types in many plant species. Thus, a key question is how embryo identity in plants is controlled, and how this process is modified during nonzygotic embryogenesis. The Arabidopsis (Arabidopsis thaliana) zygote divides to produce an embryonic lineage and an extra-embryonic suspensor. Yet, normally quiescent suspensor cells can develop a second embryo when the initial embryo is damaged, or when response to the signaling molecule auxin is locally blocked. Here we used auxin-dependent suspensor embryogenesis as a model to determine transcriptome changes during embryonic reprogramming. We found that reprogramming is complex and accompanied by large transcriptomic changes before anatomical changes. This analysis revealed a strong enrichment for genes encoding components of auxin homeostasis and response among misregulated genes. Strikingly, deregulation among multiple auxin-related gene families converged upon the re-establishment of cellular auxin levels or response. This finding points to a remarkable degree of feedback regulation to create resilience in the auxin response during embryo development. Starting from the transcriptome of auxin-deregulated embryos, we identified an auxin-dependent basic Helix Loop Helix transcription factor network that mediates the activity of this hormone in suppressing embryo development from the suspensor. |
| Databáze: | OpenAIRE |
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